PMID- 16007252
OWN - NLM
STAT- MEDLINE
DCOM- 20050831
LR  - 20220330
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 115
IP  - 7
DP  - 2005 Jul
TI  - Intrarenal cells, not bone marrow-derived cells, are the major source for 
      regeneration in postischemic kidney.
PG  - 1756-64
AB  - Ischemic injury to the kidney produces acute tubular necrosis and apoptosis 
      followed by tubular regeneration and recovery of renal function. Although mitotic 
      cells are present in the tubules of postischemic kidneys, the origins of the 
      proliferating cells are not known. Bone marrow cells (BMCs) can differentiate 
      across lineages to repair injured organs, including the kidney. However, the 
      relative contribution of intrarenal cells and extrarenal cells to kidney 
      regeneration is not clear. We produced transgenic mice that expressed enhanced 
      GFP (EGFP) specifically and permanently in mature renal tubular epithelial cells. 
      Following ischemia/reperfusion injury (IRI), EGFP-positive cells showed 
      incorporation of BrdU and expression of vimentin, which provides direct evidence 
      that the cells composing regenerating tubules are derived from renal tubular 
      epithelial cells. In BMC-transplanted mice, 89% of proliferating epithelial cells 
      originated from host cells, and 11% originated from donor BMCs. Twenty-eight days 
      after IRI, the kidneys contained 8% donor-derived cells, of which 8.4% were 
      epithelial cells, 10.6% were glomerular cells, and 81% were interstitial cells. 
      No renal functional improvement was observed in mice that were transplanted with 
      exogenous BMCs. These results show that intrarenal cells are the main source of 
      renal repair, and a single injection of BMCs does not make a significant 
      contribution to renal functional or structural recovery.
FAU - Lin, Fangming
AU  - Lin F
AD  - Department of Pediatrics and Department of Internal Medicine and Division of 
      Basic Sciences, University of Texas Southwestern Medical Center at Dallas, 
      Dallas, TX 5390-9063, USA. Fangming.lin@utsouthwestern.edu
FAU - Moran, Ashley
AU  - Moran A
FAU - Igarashi, Peter
AU  - Igarashi P
LA  - eng
GR  - K08 DK062839/DK/NIDDK NIH HHS/United States
GR  - R01 DK066535/DK/NIDDK NIH HHS/United States
GR  - DK062839/DK/NIDDK NIH HHS/United States
GR  - DK066535/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Recombinant Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
CIN - J Clin Invest. 115:1705.
MH  - Animals
MH  - Bone Marrow Cells/pathology
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Epithelial Cells/pathology
MH  - Female
MH  - Green Fluorescent Proteins/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney/injuries/*pathology/*physiology
MH  - Kidney Tubules/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Recombinant Proteins/genetics
MH  - *Regeneration
MH  - Reperfusion Injury/genetics/*pathology/physiopathology
MH  - Y Chromosome
PMC - PMC1159127
EDAT- 2005/07/12 09:00
MHDA- 2005/09/01 09:00
PMCR- 2005/07/01
CRDT- 2005/07/12 09:00
PHST- 2004/08/11 00:00 [received]
PHST- 2005/05/17 00:00 [accepted]
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2005/09/01 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
PHST- 2005/07/01 00:00 [pmc-release]
AID - 23015 [pii]
AID - 10.1172/JCI23015 [doi]
PST - ppublish
SO  - J Clin Invest. 2005 Jul;115(7):1756-64. doi: 10.1172/JCI23015.