PMID- 16008674
OWN - NLM
STAT- MEDLINE
DCOM- 20060130
LR  - 20161124
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 35
IP  - 7
DP  - 2005 Jul
TI  - Effects of various anti-asthmatic agents on mite allergen-pulsed murine bone 
      marrow-derived dendritic cells.
PG  - 884-8
AB  - BACKGROUND: Dendritic cells (DCs) play an important role in the immune response 
      and are critically involved in asthma. beta2-agonists could potentially 
      exacerbate type 2 T helper (Th2) cell-mediated immune response. OBJECTIVES: To 
      determine the effects of various anti-asthmatic agents on DCs function both in 
      vitro and in vivo. METHODS: Murine bone marrow-derived DCs were pulsed with mite 
      allergen in the presence of pranlukast, salbutamol, salmeterol or fluticasone. 
      These DCs were then inoculated intranasally into naive mice to induce allergic 
      airway inflammation in vivo. RESULTS: Pranlukast reduced IL-10 and increased 
      IL-12, while fluticasone reduced both IL-10 and IL-12 production by mite 
      allergen-pulsed DCs. Allergic airway inflammation in pranlukast- and 
      fluticasone-treated and mite allergen pulsed DCs-harbouring mice was attenuated 
      and such response was associated with inhibition of Th2 response in the airway. 
      Salbutamol did not alter cytokine production, while salmeterol reduced IL-12 
      production by mite allergen-pulsed DCs. Lung pathology in 
      beta2-agonist-harbouring mice was comparable with those of mite allergen-pulsed 
      DCs-harbouring mice. CONCLUSIONS: Our results indicate that leukotriene receptor 
      antagonists and corticosteroids inhibit DCs-induced Th2 skewed immune response, 
      and that short- and long-acting beta2-agonists do not modify DCs-induced allergic 
      airway inflammation.
FAU - Machida, I
AU  - Machida I
AD  - Second Department of Internal Medicine, Nagasaki University School of Medicine, 
      Nagasaki, Japan.
FAU - Matsuse, H
AU  - Matsuse H
FAU - Kondo, Y
AU  - Kondo Y
FAU - Kawano, T
AU  - Kawano T
FAU - Saeki, S
AU  - Saeki S
FAU - Tomari, S
AU  - Tomari S
FAU - Obase, Y
AU  - Obase Y
FAU - Fukushima, C
AU  - Fukushima C
FAU - Kohno, S
AU  - Kohno S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Androstadienes)
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Bronchodilator Agents)
RN  - 0 (Chromones)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 6EW8Q962A5 (Salmeterol Xinafoate)
RN  - CUT2W21N7U (Fluticasone)
RN  - QF8SVZ843E (Albuterol)
RN  - TB8Z891092 (pranlukast)
SB  - IM
MH  - Albuterol/analogs & derivatives/pharmacology
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Anti-Asthmatic Agents/*pharmacology
MH  - Antigens, Dermatophagoides/*immunology
MH  - Asthma/*immunology
MH  - Bone Marrow Cells/*drug effects/immunology
MH  - Bronchoconstriction/immunology
MH  - Bronchodilator Agents/pharmacology
MH  - Chromones/pharmacology
MH  - Dendritic Cells/*drug effects/immunology
MH  - Female
MH  - Fluticasone
MH  - Interleukin-10/immunology
MH  - Interleukin-12/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Respiratory System/immunology
MH  - Salmeterol Xinafoate
MH  - Th2 Cells/immunology
EDAT- 2005/07/13 09:00
MHDA- 2006/01/31 09:00
CRDT- 2005/07/13 09:00
PHST- 2005/07/13 09:00 [pubmed]
PHST- 2006/01/31 09:00 [medline]
PHST- 2005/07/13 09:00 [entrez]
AID - CEA2262 [pii]
AID - 10.1111/j.1365-2222.2005.02262.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2005 Jul;35(7):884-8. doi: 10.1111/j.1365-2222.2005.02262.x.