PMID- 16009719
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20181113
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 202
IP  - 2
DP  - 2005 Jul 18
TI  - Human CD1-restricted T cell recognition of lipids from pollens.
PG  - 295-308
AB  - Plant pollens are an important source of environmental antigens that stimulate 
      allergic responses. In addition to acting as vehicles for foreign protein 
      antigens, they contain lipids that incorporate saturated and unsaturated fatty 
      acids, which are necessary in the reproduction of higher plants. The CD1 family 
      of nonpolymorphic major histocompatibility complex-related molecules is highly 
      conserved in mammals, and has been shown to present microbial and self lipids to 
      T cells. Here, we provide evidence that pollen lipids may be recognized as 
      antigens by human T cells through a CD1-dependent pathway. Among phospholipids 
      extracted from cypress grains, phosphatidyl-choline and phosphatidyl-ethanolamine 
      were able to stimulate the proliferation of T cells from cypress-sensitive 
      subjects. Recognition of phospholipids involved multiple cell types, mostly 
      CD4(+) T cell receptor for antigen (TCR)alphabeta(+), some CD4(-)CD8(-) 
      TCRgammadelta(+), but rarely Valpha24i(+) natural killer-T cells, and required 
      CD1a(+) and CD1d(+) antigen presenting cell. The responding T cells secreted both 
      interleukin (IL)-4 and interferon-gamma, in some cases IL-10 and transforming 
      growth factor-beta, and could provide help for immunoglobulin E (IgE) production. 
      Responses to pollen phospholipids were maximally evident in blood samples 
      obtained from allergic subjects during pollinating season, uniformly absent in 
      Mycobacterium tuberculosis-exposed health care workers, but occasionally seen in 
      nonallergic subjects. Finally, allergic, but not normal subjects, displayed 
      circulating specific IgE and cutaneous weal and flare reactions to phospholipids.
FAU - Agea, Elisabetta
AU  - Agea E
AD  - Experimental Immunology and Allergy, Department of Clinical and Experimental 
      Medicine, University of Perugia, Italy.
FAU - Russano, Anna
AU  - Russano A
FAU - Bistoni, Onelia
AU  - Bistoni O
FAU - Mannucci, Roberta
AU  - Mannucci R
FAU - Nicoletti, Ildo
AU  - Nicoletti I
FAU - Corazzi, Lanfranco
AU  - Corazzi L
FAU - Postle, Anthony D
AU  - Postle AD
FAU - De Libero, Gennaro
AU  - De Libero G
FAU - Porcelli, Steven A
AU  - Porcelli SA
FAU - Spinozzi, Fabrizio
AU  - Spinozzi F
LA  - eng
GR  - R01 AI45889/AI/NIAID NIH HHS/United States
GR  - R01 AI045889/AI/NIAID NIH HHS/United States
GR  - AI48933/AI/NIAID NIH HHS/United States
GR  - R01 AI048933/AI/NIAID NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
GR  - RG0168/2000-/RG/CSR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050711
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Allergens)
RN  - 0 (Antigens, CD1)
RN  - 0 (Cytokines)
RN  - 0 (Phospholipids)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Antibody Formation/immunology
MH  - Antigen Presentation/immunology
MH  - Antigens, CD1/*immunology
MH  - Cells, Cultured
MH  - Cupressus/chemistry/*immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/immunology
MH  - Phospholipids/chemistry/*immunology
MH  - Pollen/chemistry/*immunology
MH  - Receptors, Antigen, T-Cell, gamma-delta/immunology
MH  - T-Lymphocytes/*immunology
PMC - PMC2213012
EDAT- 2005/07/13 09:00
MHDA- 2005/09/15 09:00
PMCR- 2006/01/18
CRDT- 2005/07/13 09:00
PHST- 2005/07/13 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/07/13 09:00 [entrez]
PHST- 2006/01/18 00:00 [pmc-release]
AID - jem.20050773 [pii]
AID - 20050773 [pii]
AID - 10.1084/jem.20050773 [doi]
PST - ppublish
SO  - J Exp Med. 2005 Jul 18;202(2):295-308. doi: 10.1084/jem.20050773. Epub 2005 Jul 
      11.