PMID- 16009964
OWN - NLM
STAT- MEDLINE
DCOM- 20051230
LR  - 20231213
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 11
DP  - 2005 Nov
TI  - Immunohistochemistry in human placental tissue--pitfalls of antigen detection.
PG  - 1413-20
AB  - Because incongruous controversial staining results are a common phenomenon in the 
      placenta, methodical investigations are important to prevent researchers from 
      obtaining misleading results. While investigating dendritic cells (DC) at the 
      human fetomaternal interface, we observed staining of endothelial cells (EC) in 
      chorionic villi for CD83. Given the high specificity of this antigen for DC, this 
      did not seem credible. Previous studies had revealed the same surprising staining 
      pattern with human leukocyte antigen (HLA)-G antibodies. We therefore analyzed 
      human placental EC staining more closely. Both CD83 and HLA-G antibodies were of 
      the same mouse IgG2b isotype. We also observed EC staining with a panel of 
      control antibodies of the IgG2b isotype. This suggests a high affinity of human 
      placental capillaries for mouse IgG2b. Several commonly used techniques for 
      blocking nonspecific binding of antibodies could not prevent this nonspecific EC 
      staining. A new preincubation step with purified human IgG was introduced. This 
      abolished any placental EC staining with CD83, HLA-G, and IgG2b isotype control 
      antibodies, presumably by blocking Fc receptors, whereas specific staining 
      patterns remained unchanged. Mouse antibody of the IgG2b isotype are bound 
      nonspecifically by vascular endothelial cells in human placenta and this can be 
      overcome by blocking with purified human IgG. This blocking procedure could also 
      be appropriate for frozen tissues other than placenta in which Fc receptors are 
      expressed.
FAU - Honig, Arnd
AU  - Honig A
AD  - Department of Obstetrics and Gynecology, Josef-Schneider-Strasse 4, D-97080 
      Wurzburg, Germany.
FAU - Rieger, Lorenz
AU  - Rieger L
FAU - Kapp, Michaela
AU  - Kapp M
FAU - Dietl, Johannes
AU  - Dietl J
FAU - Kammerer, Ulrike
AU  - Kammerer U
LA  - eng
PT  - Journal Article
DEP - 20050711
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Antigens)
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Fc)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Antigens/*analysis/immunology
MH  - Antigens, CD/analysis/immunology
MH  - Chorionic Villi/metabolism
MH  - Endothelial Cells/metabolism
MH  - Female
MH  - HLA Antigens/analysis/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/analysis/immunology
MH  - Humans
MH  - *Immunoglobulin G
MH  - Immunoglobulins/analysis/immunology
MH  - Immunohistochemistry/methods
MH  - Membrane Glycoproteins/analysis/immunology
MH  - Mice
MH  - Placenta/blood supply/*metabolism/ultrastructure
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Receptors, Fc/analysis
MH  - CD83 Antigen
EDAT- 2005/07/13 09:00
MHDA- 2005/12/31 09:00
CRDT- 2005/07/13 09:00
PHST- 2005/07/13 09:00 [pubmed]
PHST- 2005/12/31 09:00 [medline]
PHST- 2005/07/13 09:00 [entrez]
AID - jhc.5A6664.2005 [pii]
AID - 10.1369/jhc.5A6664.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Nov;53(11):1413-20. doi: 10.1369/jhc.5A6664.2005. Epub 
      2005 Jul 11.