PMID- 16010672
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20071203
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 234
IP  - 1
DP  - 2005 Sep
TI  - Microarray analysis of gene expression during epithelial-mesenchymal 
      transformation.
PG  - 132-42
AB  - One of the most fundamental biological processes in development, as well as a 
      primary mechanism for tumor metastasis, is epithelial-mesenchymal transformation 
      (EMT). To gain a greater understanding of this transition, we have obtained a 
      genomic profile of the critical stages before and during this rapid change in 
      morphology in the developing mouse palate. By isolating the medial edge 
      epithelium of each palatal shelf, we were able to obtain pure gene expression 
      data without contamination from surrounding mesenchymal cells. Our results 
      support the important role of the TGF-beta/Smad signal transduction pathway in 
      the stimulation of EMT by means of up-regulation of the EMT-inducing gene, LEF-1. 
      We document changes in gene expression profiles during palatal adherence and 
      subsequent transformation of the medial edge epithelial seam that suggests a high 
      number of LEF-1 target genes promote cellular transformation to mesenchyme. These 
      include genes involved in cell adhesion, polarity, cytoskeletal dynamics, 
      migration, and intracellular signaling. This knowledge of the changes in gene 
      expression levels during palatogenesis should lead to a better understanding of 
      the mechanisms of EMT.
CI  - Copyright 2005 Wiley-Liss, Inc.
FAU - LaGamba, Damian
AU  - LaGamba D
AD  - Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
FAU - Nawshad, Ali
AU  - Nawshad A
FAU - Hay, Elizabeth D
AU  - Hay ED
LA  - eng
SI  - OMIM/GSE2537
GR  - R01-DE11142/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Epithelium/embryology/metabolism
MH  - Gene Expression Profiling
MH  - Mesoderm/cytology/*metabolism
MH  - Mice
MH  - Oligonucleotide Array Sequence Analysis
MH  - Palate/cytology/embryology/*metabolism
MH  - Signal Transduction/physiology
MH  - Transforming Growth Factor beta/physiology
EDAT- 2005/07/13 09:00
MHDA- 2007/05/09 09:00
CRDT- 2005/07/13 09:00
PHST- 2005/07/13 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2005/07/13 09:00 [entrez]
AID - 10.1002/dvdy.20489 [doi]
PST - ppublish
SO  - Dev Dyn. 2005 Sep;234(1):132-42. doi: 10.1002/dvdy.20489.