PMID- 16010692
OWN - NLM
STAT- MEDLINE
DCOM- 20060417
LR  - 20191210
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 20
IP  - 1
DP  - 2006 Jan
TI  - Determination of diclofenac in plasma by high-performance liquid chromatography 
      with electrochemical detection.
PG  - 119-24
AB  - A reversed-phase high-performance liquid chromatographic method with 
      electrochemical detection for the quantitative determination of diclofenac 
      potassium in plasma was developed. Naproxen was used as the internal standard. 
      The drug and internal standard were isolated from plasma by extraction with 
      dichloromethane and 2 M hydrochloric acid. Chromatographic separation was 
      performed on a C18 column with methanol-water (68:32, v/v) adjusted to pH 3.2 
      with phosphoric acid as mobile phase. The oxidation potential for detection was 
      established by constructing a voltammogram for diclofenac. The quantification 
      limit for diclofenac in plasma was 5 ng mL(-1). Linearity of the method was 
      confirmed in the range 5-2000 ng mL(-1), correlation coefficient 0.9998. 
      Within-day relative standard deviations (RSDs) ranged from 0.66 to 14.00% and 
      between-day RSDs from 0.59 to 15.78%. The method was successfully applied for the 
      determination of pharmacokinetic parameters after ingestion of a 50 mg dose of 
      diclofenac. Studies were performed on 18 healthy volunteers of both sexes.
CI  - Copyright 2005 John Wiley & Sons, Ltd.
FAU - Chmielewska, Aleksandra
AU  - Chmielewska A
AD  - Medical University of Gdansk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdansk, 
      Poland. chmola@amg.gda.pl
FAU - Konieczna, Lucyna
AU  - Konieczna L
FAU - Plenis, Alina
AU  - Plenis A
FAU - Bieniecki, Michal
AU  - Bieniecki M
FAU - Lamparczyk, Henryk
AU  - Lamparczyk H
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 144O8QL0L1 (Diclofenac)
SB  - IM
MH  - Adult
MH  - Anti-Inflammatory Agents, Non-Steroidal/*blood/pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Diclofenac/*blood/pharmacokinetics
MH  - Electrochemistry/*methods
MH  - Female
MH  - Humans
MH  - Male
MH  - Reference Standards
MH  - Reproducibility of Results
EDAT- 2005/07/13 09:00
MHDA- 2006/04/18 09:00
CRDT- 2005/07/13 09:00
PHST- 2005/07/13 09:00 [pubmed]
PHST- 2006/04/18 09:00 [medline]
PHST- 2005/07/13 09:00 [entrez]
AID - 10.1002/bmc.537 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2006 Jan;20(1):119-24. doi: 10.1002/bmc.537.