PMID- 16012934
OWN - NLM
STAT- MEDLINE
DCOM- 20050929
LR  - 20220330
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 129
IP  - 1
DP  - 2005 Jul
TI  - Characteristics of intestinal dendritic cells in inflammatory bowel diseases.
PG  - 50-65
AB  - BACKGROUND & AIMS: Dendritic cells (DCs) recognize and respond to microbial 
      structures using pattern recognition receptors, including Toll-like receptors 
      (TLRs). In the intestine, DCs are pivotal in tolerance induction and direct the 
      differentiation of T cells. We aimed to identify changes in intestinal DCs that 
      may underlie the dysregulated immune response to enteric bacteria that occurs in 
      patients with inflammatory bowel disease (IBD). METHODS: DCs were identified in 
      freshly isolated lamina propria mononuclear cells by multicolor flow cytometry in 
      patients with IBD and controls. Expression of TLR2, TLR4, and the 
      activation/maturation marker CD40 was assessed by cell surface labeling. 
      Production of cytokines (interleukin [IL]-12, IL-6, and IL-10) was assessed in 
      the absence of exogenous stimulation by intracellular staining of permeabilized 
      cells. RESULTS: In healthy controls, few intestinal DCs expressed TLR2 or TLR4, 
      in contrast to blood DCs. DC expression of both TLRs was significantly enhanced 
      in Crohn's disease and ulcerative colitis. DCs from inflamed tissue of patients 
      with Crohn's disease expressed significantly higher levels of the 
      maturation/activation marker CD40. Elevated levels of CD40 on DCs were decreased 
      after treating patients with anti-tumor necrosis factor alpha. In Crohn's 
      disease, but not ulcerative colitis, more colonic DCs produced IL-12 and IL-6. 
      The number of IL-10-producing DCs did not differ significantly between patients 
      with IBD and controls. CONCLUSIONS: In IBD, DCs are activated, their expression 
      of microbial recognition receptors is up-regulated, and more DCs produce 
      pathologically relevant cytokines. Intestinal DCs are likely to be key initiators 
      or perpetuators of the inflammatory response that characterizes IBD.
FAU - Hart, Ailsa L
AU  - Hart AL
AD  - Antigen Presentation Research Group, Imperial College London, England.
FAU - Al-Hassi, Hafid Omar
AU  - Al-Hassi HO
FAU - Rigby, Rachael J
AU  - Rigby RJ
FAU - Bell, Sally J
AU  - Bell SJ
FAU - Emmanuel, Anton V
AU  - Emmanuel AV
FAU - Knight, Stella C
AU  - Knight SC
FAU - Kamm, Michael A
AU  - Kamm MA
FAU - Stagg, Andrew J
AU  - Stagg AJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Biomarkers)
RN  - 0 (CD40 Antigens)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers
MH  - CD40 Antigens/metabolism
MH  - Colon/cytology/*immunology
MH  - Crohn Disease/*immunology/*pathology
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Middle Aged
MH  - Receptors, Cell Surface/metabolism
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 2005/07/14 09:00
MHDA- 2005/09/30 09:00
CRDT- 2005/07/14 09:00
PHST- 2005/07/14 09:00 [pubmed]
PHST- 2005/09/30 09:00 [medline]
PHST- 2005/07/14 09:00 [entrez]
AID - S0016508505008838 [pii]
AID - 10.1053/j.gastro.2005.05.013 [doi]
PST - ppublish
SO  - Gastroenterology. 2005 Jul;129(1):50-65. doi: 10.1053/j.gastro.2005.05.013.