PMID- 16013231
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 22
IP  - 3
DP  - 2005 Jun
TI  - [Polymeric nanoparticles with therapeutic gene for gene therapy: I. Preparation 
      and in vivo gene transfer study].
PG  - 438-42
AB  - VEGF nanoparticle (VEGF-NP) was prepared by a multi-emulsification technique 
      using a biodegradable poly-dl-lactic-co-glycolic (PLGA) as matrix material. The 
      nanoparticles were characterized for size, VEGF loading capacity, and in vitro 
      release. VEGF-NP and naked VEGF plasmid were intramuscularly injected into the 
      ischemia site of the rabbit chronic hindlimb ischemia model and the efficiency of 
      VEGF-NP as gene delivery carrier for gene therapy in animal model was evaluated. 
      Gene therapuetic effect was assessed evaluated by RT-PCR, immunohistochemistry 
      and angiography assay. The average size of VEGF-NP was around 300 nm. The 
      encapsulation efficiency of VEGF was above 96%. Loading amount of VEGF in the 
      nanoparticles was about 4%. In vitro, nanoparticles maintained sustained-release 
      of VEGF for two weeks. Two weeks post gene injection the capillary density in 
      VEGF-NP group (81.22 per mm2) was significantly higher than that in control group 
      (29.54 mm2). RT-PCR results showed greatly higher VEGF expression in VEGF-NP 
      group (31.79au * mm) than that in naked VEGF group (9.15 au * mm). As a carrier 
      system for gene therapy in animal model, VEGF-NP is much better than naked DNA 
      plasmid. The results demonstrate great possibility of using NP carrier in human 
      gene therapy.
FAU - Yang, Jing
AU  - Yang J
AD  - Institute of Biomedical Engineering, CAMS & PUMC, Tianjin 300192, China.
FAU - Song, Cunxian
AU  - Song C
FAU - Sun, Hongfan
AU  - Sun H
FAU - Wu, Li
AU  - Wu L
FAU - Tang, Lina
AU  - Tang L
FAU - Leng, Xigang
AU  - Leng X
FAU - Wang, Pengyan
AU  - Wang P
FAU - Xu, Yiyao
AU  - Xu Y
FAU - Li, Yongjun
AU  - Li Y
FAU - Guan, Heng
AU  - Guan H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Gene Transfer Techniques
MH  - *Genetic Therapy
MH  - Genetic Vectors/chemistry
MH  - Lactic Acid/*chemistry
MH  - Nanoparticles/*chemistry
MH  - Plasmids
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rabbits
MH  - Vascular Endothelial Growth Factor A/*genetics
EDAT- 2005/07/15 09:00
MHDA- 2015/05/20 06:00
CRDT- 2005/07/15 09:00
PHST- 2005/07/15 09:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PHST- 2005/07/15 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Jun;22(3):438-42.