PMID- 16013507
OWN - NLM
STAT- MEDLINE
DCOM- 20060714
LR  - 20140625
VI  - 19
IP  - 6
DP  - 2005 Mar
TI  - [Methylation of promoter and expression of FHIT gene in laryngeal squamous cell 
      carcinoma].
PG  - 253-5, 258
AB  - OBJECTIVE: To study the relationship between status of methylation of FHIT gene 
      promoter and expression of its protein in laryngeal squamous cell carcinoma 
      (LSCC). METHOD: PCR-based restriction enzyme assay and immunohistochemical SP 
      technique were used to detect the status of methylation of FHIT gene promoter and 
      expression of its protein in 41 cases of LSCC and their matched normal squamous 
      epithelium (NSE). RESULT: In NSE, there was no promoter methylation of FHIT gene, 
      while in LSCC the rate of it was 24.4% (10/41), and it was related to the tumor 
      pathological grade and lymph node metastasis (P < 0.01). The positive rate of 
      FHIT expression in NSE and LSCC was 100.0% (41/41) and 46.3% (19/41) 
      respectively, and the expression of FHIT was related to the tumor TNM stage and 
      lymph node metastasis (P < 0.05). There was significant correlation between 
      methylation of FHIT gene promoter and expression of its protein (P < 0.01). 
      CONCLUSION: Methylation of promoter might be one of important factors of 
      inactivation of FHIT gene, and might play important role in carcinogenesis and 
      progression of LSCC.
FAU - Yin, Detao
AU  - Yin D
AD  - Department of Otolaryngology-Head and Neck Surgery, the First Affiliated Hospital 
      of Zhengzhou University, Zhengzhou, 450052, China. detaoyin@zzu.edu.cn
FAU - Dong, Mingmin
AU  - Dong M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Ke Za Zhi
JT  - Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
JID - 9426080
RN  - 0 (Neoplasm Proteins)
RN  - 0 (fragile histidine triad protein)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
SB  - IM
MH  - Acid Anhydride Hydrolases/*biosynthesis/genetics
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*biosynthesis/genetics
EDAT- 2005/07/15 09:00
MHDA- 2006/07/15 09:00
CRDT- 2005/07/15 09:00
PHST- 2005/07/15 09:00 [pubmed]
PHST- 2006/07/15 09:00 [medline]
PHST- 2005/07/15 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Mar;19(6):253-5, 258.