PMID- 16013567
OWN - NLM
STAT- MEDLINE
DCOM- 20100602
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 23
IP  - 2
DP  - 2005 Mar
TI  - [Study on characteristics of bias caused by flow injection-capillary 
      electrophoresis with split-flow electrokinetic injection].
PG  - 189-92
AB  - The characteristics of bias caused by split-flow electrokinetic injection (SEKI), 
      a new sample injection method applied in coupled flow injection-capillary 
      electrophoretic system (FI-CE), was investigated using pseudoephedrine 
      hydrochloride, a basic drug and ibuprofen, an acidic drug, as model analytes. It 
      was found that bias imposed by SEKI under the conditions of continuous sample 
      matrix/running buffer was similar to that did by electrokinetic injection (EKI). 
      The linearity of calibration curve provided by SEKI was similar to that offered 
      by non-bias hydrodynamic injection (HDI) but significantly better than that 
      obtained by EKI. These features were exploited to improve analytical performances 
      in simultaneous determination of the minor ingredient of pseudoephedrine 
      hydrochloride and the major ingredient of ibuprofen in pharmaceutical 
      preparation. Detectability of 0.7 mg/L for pseudoephedrine hydrochloride, was 
      achieved at a sample throughput rate of 24 times per hour which is 30% lower than 
      that obtained by HDI-based conventional CE. Eleven runs of a test solution 
      containing 13.1 mg/L pseudoephedrine hydrochloride and 81.4 mg/L ibuprofen 
      produced relative standard deviations (RSDs) of 2.8% for the minor ingredient and 
      1.2% for the major ingredient, which were improved in comparision to that 
      obtained by HDI-based conventional CE. Analytical results for two batches of 
      compound ibuprofen tablets by the SEKI-based FI-CE approach were in good 
      agreement with that obtained by a conventional high performance liquid 
      chromatographic method.
FAU - Luo, Jinwen
AU  - Luo J
AD  - Zhejiang Institute for Drug Control, Hangzhou 310004, China. liwzhl@hzcnc.com
FAU - Zhu, Hailin
AU  - Zhu H
FAU - Li, Huilin
AU  - Li H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Bronchodilator Agents)
RN  - 7CUC9DDI9F (Pseudoephedrine)
RN  - WK2XYI10QM (Ibuprofen)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*analysis/chemistry
MH  - Bronchodilator Agents/*analysis/chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Flow Injection Analysis/*methods
MH  - Ibuprofen/*analysis/chemistry
MH  - Pseudoephedrine/*analysis/chemistry
EDAT- 2005/07/15 09:00
MHDA- 2010/06/03 06:00
CRDT- 2005/07/15 09:00
PHST- 2005/07/15 09:00 [pubmed]
PHST- 2010/06/03 06:00 [medline]
PHST- 2005/07/15 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2005 Mar;23(2):189-92.