PMID- 16014907
OWN - NLM
STAT- MEDLINE
DCOM- 20050818
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 15
DP  - 2005 Aug
TI  - Variant-specific tropism of human herpesvirus 6 in human astrocytes.
PG  - 9439-48
AB  - Though first described as a lymphotropic virus, human herpesvirus 6 (HHV-6) is 
      highly neuropathogenic. Two viral variants are known: HHV-6A and HHV-6B. Both 
      variants can infect glial cells and have been differentially associated with 
      central nervous system diseases, suggesting an HHV-6 variant-specific tropism for 
      glial cell subtypes. We have performed infections with both viral variants in 
      human progenitor-derived astrocytes (HPDA) and monitored infected cell cultures 
      for cytopathic effect (CPE), intra- and extracellular viral DNA load, the 
      presence of viral particles by electronic microscopy, mRNA transcription, and 
      viral protein expression. HHV-6A established a productive infection with CPE, 
      visible intracellular virions, and high virus DNA loads. HHV-6B-infected HPDA 
      showed no morphological changes, intracellular viral particles, and decreasing 
      intra- and extracellular viral DNA over time. After long-term passage, 
      HHV-6B-infected HPDA had stable but low levels of intracellular viral DNA load 
      with no detectable viral mRNA. Our results demonstrate that HHV-6A and HHV-6B 
      have differential tropisms and patterns of infection for HPDA in vitro, where 
      HHV-6A results in a productive lytic infection. In contrast, HHV-6B was 
      associated with a nonproductive infection. These findings suggest that HHV-6 
      variants might be responsible for specific infection patterns in glial cells in 
      vivo. Astrocytes may be an important reservoir for this virus in which 
      differential tropism of HHV-6A and HHV-6B may be associated with different 
      disease outcomes.
FAU - Donati, Donatella
AU  - Donati D
AD  - Viral Immunology Section, National Institute of Neurological Disorders and 
      Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Martinelli, Elena
AU  - Martinelli E
FAU - Cassiani-Ingoni, Riccardo
AU  - Cassiani-Ingoni R
FAU - Ahlqvist, Jenny
AU  - Ahlqvist J
FAU - Hou, Jean
AU  - Hou J
FAU - Major, Eugene O
AU  - Major EO
FAU - Jacobson, Steve
AU  - Jacobson S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Astrocytes/*virology
MH  - Cells, Cultured
MH  - Cytopathogenic Effect, Viral
MH  - Herpesvirus 6, Human/growth & development/*physiology
MH  - Humans
MH  - Roseolovirus Infections/*virology
MH  - Species Specificity
MH  - Virus Cultivation
PMC - PMC1181567
EDAT- 2005/07/15 09:00
MHDA- 2005/08/19 09:00
PMCR- 2005/08/01
CRDT- 2005/07/15 09:00
PHST- 2005/07/15 09:00 [pubmed]
PHST- 2005/08/19 09:00 [medline]
PHST- 2005/07/15 09:00 [entrez]
PHST- 2005/08/01 00:00 [pmc-release]
AID - 79/15/9439 [pii]
AID - 0252-05 [pii]
AID - 10.1128/JVI.79.15.9439-9448.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 Aug;79(15):9439-48. doi: 10.1128/JVI.79.15.9439-9448.2005.