PMID- 1601643
OWN - NLM
STAT- MEDLINE
DCOM- 19920713
LR  - 20190918
IS  - 0162-3109 (Print)
IS  - 0162-3109 (Linking)
VI  - 23
IP  - 2
DP  - 1992 Mar-Apr
TI  - Interleukin-6 can prime THP-1 macrophages for enhanced production of tumor 
      necrosis factor-alpha in response to LPS.
PG  - 97-103
AB  - Although interferon-gamma has been shown to effectively prime macrophages for 
      enhanced production of tumor necrosis factor-alpha (TNF alpha), it is reasonable 
      to assume that other cytokines present in the extracellular environment may 
      likewise facilitate cytokine biosynthesis. For example, interleukin-6 (IL-6) is 
      synthesized by synovial lining macrophages and fibroblasts, and has been detected 
      (along with TNF alpha) in rheumatoid synovial effusions. Therefore, the purpose 
      of the present study was to determine whether IL-6 influences the production of 
      IL-1 beta and/or TNF alpha by THP-1 macrophages. Although IL-6 treatment alone 
      resulted in only a slight increase in TNF alpha levels, administration of IL-6 
      followed by Sal. minnesota LPS resulted in a synergistic potentiation of TNF 
      alpha production by THP-1 macrophages. The priming effect of IL-6 could be 
      reversed by boiling, or by the addition of a neutralizing polyclonal antibody 
      against IL-6. Notably, IL-6 only weakly enhanced interleukin-1 beta production. 
      In summary, the ability of IL-6 to potentiate TNF alpha production by THP-1 
      macrophages may provide insight into the regulation of the cytokine network in 
      inflammatory diseases, such as rheumatoid arthritis.
FAU - Cochran, F R
AU  - Cochran FR
AD  - Department of Allergy and Inflammation, Hoffmann-LaRoche, Nutley, NJ 07110.
FAU - Finch-Arietta, M B
AU  - Finch-Arietta MB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunopharmacology
JT  - Immunopharmacology
JID - 7902474
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Arthritis, Rheumatoid/etiology
MH  - Cell Line
MH  - Drug Synergism
MH  - Humans
MH  - Interleukin-1/biosynthesis
MH  - Interleukin-6/administration & dosage/*pharmacology
MH  - Lipopolysaccharides/administration & dosage
MH  - Macrophage Activation
MH  - Macrophages/*immunology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 0162-3109(92)90033-9 [pii]
AID - 10.1016/0162-3109(92)90033-9 [doi]
PST - ppublish
SO  - Immunopharmacology. 1992 Mar-Apr;23(2):97-103. doi: 10.1016/0162-3109(92)90033-9.