PMID- 16020725 OWN - NLM STAT- MEDLINE DCOM- 20050726 LR - 20231127 IS - 1095-9203 (Electronic) IS - 0036-8075 (Linking) VI - 309 IP - 5733 DP - 2005 Jul 15 TI - The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. PG - 409-15 AB - Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention. FAU - El-Sayed, Najib M AU - El-Sayed NM AD - Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA. nelsayed@tigr.org FAU - Myler, Peter J AU - Myler PJ FAU - Bartholomeu, Daniella C AU - Bartholomeu DC FAU - Nilsson, Daniel AU - Nilsson D FAU - Aggarwal, Gautam AU - Aggarwal G FAU - Tran, Anh-Nhi AU - Tran AN FAU - Ghedin, Elodie AU - Ghedin E FAU - Worthey, Elizabeth A AU - Worthey EA FAU - Delcher, Arthur L AU - Delcher AL FAU - Blandin, Gaelle AU - Blandin G FAU - Westenberger, Scott J AU - Westenberger SJ FAU - Caler, Elisabet AU - Caler E FAU - Cerqueira, Gustavo C AU - Cerqueira GC FAU - Branche, Carole AU - Branche C FAU - Haas, Brian AU - Haas B FAU - Anupama, Atashi AU - Anupama A FAU - Arner, Erik AU - Arner E FAU - Aslund, Lena AU - Aslund L FAU - Attipoe, Philip AU - Attipoe P FAU - Bontempi, Esteban AU - Bontempi E FAU - Bringaud, Frederic AU - Bringaud F FAU - Burton, Peter AU - Burton P FAU - Cadag, Eithon AU - Cadag E FAU - Campbell, David A AU - Campbell DA FAU - Carrington, Mark AU - Carrington M FAU - Crabtree, Jonathan AU - Crabtree J FAU - Darban, Hamid AU - Darban H FAU - da Silveira, Jose Franco AU - da Silveira JF FAU - de Jong, Pieter AU - de Jong P FAU - Edwards, Kimberly AU - Edwards K FAU - Englund, Paul T AU - Englund PT FAU - Fazelina, Gholam AU - Fazelina G FAU - Feldblyum, Tamara AU - Feldblyum T FAU - Ferella, Marcela AU - Ferella M FAU - Frasch, Alberto Carlos AU - Frasch AC FAU - Gull, Keith AU - Gull K FAU - Horn, David AU - Horn D FAU - Hou, Lihua AU - Hou L FAU - Huang, Yiting AU - Huang Y FAU - Kindlund, Ellen AU - Kindlund E FAU - Klingbeil, Michele AU - Klingbeil M FAU - Kluge, Sindy AU - Kluge S FAU - Koo, Hean AU - Koo H FAU - Lacerda, Daniela AU - Lacerda D FAU - Levin, Mariano J AU - Levin MJ FAU - Lorenzi, Hernan AU - Lorenzi H FAU - Louie, Tin AU - Louie T FAU - Machado, Carlos Renato AU - Machado CR FAU - McCulloch, Richard AU - McCulloch R FAU - McKenna, Alan AU - McKenna A FAU - Mizuno, Yumi AU - Mizuno Y FAU - Mottram, Jeremy C AU - Mottram JC FAU - Nelson, Siri AU - Nelson S FAU - Ochaya, Stephen AU - Ochaya S FAU - Osoegawa, Kazutoyo AU - Osoegawa K FAU - Pai, Grace AU - Pai G FAU - Parsons, Marilyn AU - Parsons M FAU - Pentony, Martin AU - Pentony M FAU - Pettersson, Ulf AU - Pettersson U FAU - Pop, Mihai AU - Pop M FAU - Ramirez, Jose Luis AU - Ramirez JL FAU - Rinta, Joel AU - Rinta J FAU - Robertson, Laura AU - Robertson L FAU - Salzberg, Steven L AU - Salzberg SL FAU - Sanchez, Daniel O AU - Sanchez DO FAU - Seyler, Amber AU - Seyler A FAU - Sharma, Reuben AU - Sharma R FAU - Shetty, Jyoti AU - Shetty J FAU - Simpson, Anjana J AU - Simpson AJ FAU - Sisk, Ellen AU - Sisk E FAU - Tammi, Martti T AU - Tammi MT FAU - Tarleton, Rick AU - Tarleton R FAU - Teixeira, Santuza AU - Teixeira S FAU - Van Aken, Susan AU - Van Aken S FAU - Vogt, Christy AU - Vogt C FAU - Ward, Pauline N AU - Ward PN FAU - Wickstead, Bill AU - Wickstead B FAU - Wortman, Jennifer AU - Wortman J FAU - White, Owen AU - White O FAU - Fraser, Claire M AU - Fraser CM FAU - Stuart, Kenneth D AU - Stuart KD FAU - Andersson, Bjorn AU - Andersson B LA - eng GR - R01 AI031077/AI/NIAID NIH HHS/United States GR - AI45061/AI/NIAID NIH HHS/United States GR - AI045039/AI/NIAID NIH HHS/United States GR - R01 AI031077-11/AI/NIAID NIH HHS/United States GR - U01 AI045039/AI/NIAID NIH HHS/United States GR - AI45038/AI/NIAID NIH HHS/United States GR - U01 AI045038/AI/NIAID NIH HHS/United States GR - Wellcome Trust/United Kingdom PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 RN - 0 (DNA, Mitochondrial) RN - 0 (DNA, Protozoan) RN - 0 (Membrane Proteins) RN - 0 (Protozoan Proteins) RN - 0 (Retroelements) RN - 0 (Trypanocidal Agents) SB - IM MH - Animals MH - Chagas Disease/drug therapy/parasitology MH - DNA Repair MH - DNA Replication MH - DNA, Mitochondrial/genetics MH - DNA, Protozoan/genetics MH - Genes, Protozoan MH - *Genome, Protozoan MH - Humans MH - Meiosis MH - Membrane Proteins/chemistry/genetics/physiology MH - Multigene Family MH - Protozoan Proteins/chemistry/*genetics/physiology MH - Recombination, Genetic MH - Repetitive Sequences, Nucleic Acid MH - Retroelements MH - *Sequence Analysis, DNA MH - Signal Transduction MH - Telomere/genetics MH - Trypanocidal Agents/pharmacology/therapeutic use MH - Trypanosoma cruzi/chemistry/*genetics/physiology EDAT- 2005/07/16 09:00 MHDA- 2005/07/27 09:00 CRDT- 2005/07/16 09:00 PHST- 2005/07/16 09:00 [pubmed] PHST- 2005/07/27 09:00 [medline] PHST- 2005/07/16 09:00 [entrez] AID - 309/5733/409 [pii] AID - 10.1126/science.1112631 [doi] PST - ppublish SO - Science. 2005 Jul 15;309(5733):409-15. doi: 10.1126/science.1112631.