PMID- 16023680 OWN - NLM STAT- MEDLINE DCOM- 20060801 LR - 20161124 IS - 0025-326X (Print) IS - 0025-326X (Linking) VI - 51 IP - 8-12 DP - 2005 TI - cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus. PG - 769-75 AB - We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125-129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay. FAU - Lee, Young-Mi AU - Lee YM AD - Department of Environmental Science, Graduate School, Hanyang University, Seoul 133-791, South Korea. FAU - Williams, Tim D AU - Williams TD FAU - Jung, Sang-Oun AU - Jung SO FAU - Lee, Jae-Seong AU - Lee JS LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20050714 PL - England TA - Mar Pollut Bull JT - Marine pollution bulletin JID - 0260231 RN - 0 (DNA, Complementary) RN - 0 (Phenols) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1A1) RN - I03GBV4WEL (4-nonylphenol) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cloning, Molecular MH - Cluster Analysis MH - Cyprinodontiformes/*genetics MH - Cytochrome P-450 CYP1A1/*genetics/*metabolism MH - DNA, Complementary/genetics MH - Enzyme Induction/drug effects MH - Molecular Sequence Data MH - Phenols/toxicity MH - *Phylogeny MH - Sequence Alignment MH - Sequence Analysis, DNA EDAT- 2005/07/19 09:00 MHDA- 2006/08/02 09:00 CRDT- 2005/07/19 09:00 PHST- 2005/07/19 09:00 [pubmed] PHST- 2006/08/02 09:00 [medline] PHST- 2005/07/19 09:00 [entrez] AID - S0025-326X(05)00229-8 [pii] AID - 10.1016/j.marpolbul.2005.06.015 [doi] PST - ppublish SO - Mar Pollut Bull. 2005;51(8-12):769-75. doi: 10.1016/j.marpolbul.2005.06.015. Epub 2005 Jul 14.