PMID- 16024918
OWN - NLM
STAT- MEDLINE
DCOM- 20060112
LR  - 20220227
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 46
IP  - 10
DP  - 2005 Oct
TI  - The human ABCG1 gene: identification of LXR response elements that modulate 
      expression in macrophages and liver.
PG  - 2151-67
AB  - The ABC transporter ABCG1 (ATP binding cassette transporter G1), expressed in 
      macrophages, liver, and other tissues, has been implicated in the efflux of 
      cholesterol to high density lipoprotein. The ABCG1 gene is transcriptionally 
      activated by cholesterol loading and activators of liver X receptors (LXRs) and 
      retinoid X receptors (RXRs) through genomic sequences that have not been fully 
      characterized. Here we show that ABCG1 mRNA is induced by LXR agonists in 
      RAW264.7 macrophage cells, HepG2 hepatoma cells, and primary mouse hepatocytes. 
      We identify two evolutionarily highly conserved LXR response elements (LXREs), 
      LXRE-A and LXRE-B, located in the first and second introns of the human ABCG1 
      gene. Each element conferred robust LXR-agonist responsiveness to ABCG1 
      promoter-directed luciferase gene constructs in RAW264.7 and HepG2 cells. 
      Overexpression of LXR/RXR activated the ABCG1 promoter in the presence of LXRE-A 
      or LXRE-B sequences. In gel-shift assays, LXR/RXR heterodimers bound to wild-type 
      but not to mutated LXRE-A and LXRE-B sequences. In chromatin immunoprecipitation 
      assays, LXR and RXR were detected at LXRE-A and -B regions of DNA of human THP-1 
      macrophages. These studies clarify the mechanism of transcriptional upregulation 
      of the ABCG1 gene by oxysterols in macrophages and liver, two key tissues where 
      ABCG1 expression may affect cholesterol balance and atherogenesis.
FAU - Sabol, Steven L
AU  - Sabol SL
AD  - Molecular Disease Branch, National Heart, Lung, and Blood Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA. slsabol@mail.nih.gov
FAU - Brewer, H Bryan Jr
AU  - Brewer HB Jr
FAU - Santamarina-Fojo, Silvia
AU  - Santamarina-Fojo S
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050716
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hydrocarbons, Fluorinated)
RN  - 0 (Liver X Receptors)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Sulfonamides)
RN  - 0 (T0901317)
RN  - 1UA8E65KDZ (Alitretinoin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/*genetics
MH  - Alitretinoin
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/agonists/*genetics
MH  - Electrophoretic Mobility Shift Assay
MH  - Hepatocytes/*metabolism
MH  - Humans
MH  - Hydrocarbons, Fluorinated
MH  - Liver X Receptors
MH  - Macrophages/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Orphan Nuclear Receptors
MH  - Receptors, Cytoplasmic and Nuclear/agonists/*genetics
MH  - Response Elements/*genetics
MH  - Retinoid X Receptors/agonists
MH  - Sequence Alignment
MH  - Sulfonamides/pharmacology
MH  - Transcription Initiation Site
MH  - Tretinoin/pharmacology
MH  - Up-Regulation
EDAT- 2005/07/19 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/07/19 09:00
PHST- 2005/07/19 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/07/19 09:00 [entrez]
AID - S0022-2275(20)32906-0 [pii]
AID - 10.1194/jlr.M500080-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2005 Oct;46(10):2151-67. doi: 10.1194/jlr.M500080-JLR200. Epub 2005 
      Jul 16.