PMID- 16025312
OWN - NLM
STAT- MEDLINE
DCOM- 20060522
LR  - 20061115
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 79
IP  - 12
DP  - 2005 Dec
TI  - The local lymph node assay being too sensitive?
PG  - 721-8
AB  - The local lymph node assay (LLNA) and modifications thereof were recently 
      recognized by the OECD as stand-alone methods for the detection of 
      skin-sensitizing potential. However, although the validity of the LLNA was 
      acknowledged by the ICCVAM, attention was drawn to one major problem, i.e., the 
      possibility of false positive results caused by non-specific cell activation as a 
      result of inflammatory processes in the skin (irritation). This is based on the 
      fact that inflammatory processes in the skin may lead to non-specific activation 
      of dendritic cells, cell migration and non-specific proliferation of lymph node 
      cells. Measuring cell proliferation by radioactive or non-radioactive methods, 
      without taking the irritating properties of test items into account, leads thus 
      to false positive reactions. In this paper, we have compared both endpoints: (1) 
      cell proliferation alone and (2) cell proliferation in combination with 
      inflammatory (irritating) processes. It turned out that a considerable number of 
      tests were "false positive" to the definition mentioned above. By excluding such 
      false positive results the LLNA seems not to be more sensitive than relevant 
      guinea pig assays. These various methods and results are described here.
FAU - Hans-Werner, Vohr
AU  - Hans-Werner V
AD  - Toxicology, Bayer HealthCare AG, Aprather Weg, 42096 Wuppertal, Germany. 
      Hans-werner.vohr@bayerhealthcare.com
FAU - Jurgen, Ahr Hans
AU  - Jurgen AH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20050716
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Allergens)
RN  - 0 (Irritants)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Dermatitis, Allergic Contact
MH  - False Positive Reactions
MH  - Female
MH  - Guinea Pigs
MH  - Irritants/*toxicity
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred Strains
MH  - *Patch Tests
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2005/07/19 09:00
MHDA- 2006/05/23 09:00
CRDT- 2005/07/19 09:00
PHST- 2005/04/06 00:00 [received]
PHST- 2005/05/18 00:00 [accepted]
PHST- 2005/07/19 09:00 [pubmed]
PHST- 2006/05/23 09:00 [medline]
PHST- 2005/07/19 09:00 [entrez]
AID - 10.1007/s00204-005-0005-9 [doi]
PST - ppublish
SO  - Arch Toxicol. 2005 Dec;79(12):721-8. doi: 10.1007/s00204-005-0005-9. Epub 2005 
      Jul 16.