PMID- 16025461
OWN - NLM
STAT- MEDLINE
DCOM- 20050927
LR  - 20061115
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 489
IP  - 4
DP  - 2005 Sep 5
TI  - 3D atlas describing the ontogenic evolution of the primary olfactory projections 
      in the olfactory bulb of Xenopus laevis.
PG  - 403-24
AB  - The adult Xenopus presents the unique capability to smell odors both in water and 
      air thanks to two different olfactory pathways. Nevertheless, the tadpole can 
      initially perceive only water-borne odorants, as the olfactory receptor neurons 
      (ORN) that will detect air-borne odorants develop later. Such a phenomenon 
      requires major reorganization processes. Here we focused on the precise 
      description of the neuroanatomical modifications occurring in the olfactory bulb 
      (OB) of the tadpole throughout metamorphosis. Using both carbocyanine dyes and 
      lectin staining, we investigated the evolution of ORN projection patterns into 
      the OB from Stages 47 to 66, thus covering the period of time when all the 
      modifications take place. Although our results confirm previous works (Reiss and 
      Burd [1997] Semin Cell Dev Biol 8:171-179), we showed for the first time that the 
      main olfactory bulb (MOB) is subdivided into seven zones at Stage 47 plus the 
      accessory olfactory bulb (AOB). These seven zones receive fibers dedicated to 
      aquatic olfaction ("aquatic fibers") and are conserved until Stage 66. At Stage 
      48 the first fibers dedicated to the aerial olfaction constitute a new 
      dorsomedial zone that grows steadily, pushing the seven original zones 
      ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, 
      reminiscent of the organization described in fish. This raises the question of 
      whether such an organization in zones constitutes a plesiomorphy or is linked to 
      aquatic olfaction. We generated a 3D atlas at several stages which are 
      representative of the reorganization process. This will be a useful tool for 
      future studies of development and function.
FAU - Gaudin, Arnaud
AU  - Gaudin A
AD  - Centre des Sciences du Gout (Unite Mixte de Recherche 5170 Centre National de la 
      Recherche Scientifique-Universite de Bourgogne-Institut National de la Recherche 
      Agronomique), F-21000 Dijon, France.
FAU - Gascuel, Jean
AU  - Gascuel J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lectins)
SB  - IM
MH  - Animals
MH  - Brain Mapping
MH  - Carbocyanines
MH  - Female
MH  - Fluorescent Dyes
MH  - Growth Cones/physiology
MH  - Larva/anatomy & histology/growth & development
MH  - Lectins
MH  - Male
MH  - Metamorphosis, Biological/physiology
MH  - Microscopy, Confocal
MH  - Olfactory Bulb/anatomy & histology/*growth & development
MH  - Olfactory Nerve/anatomy & histology/*growth & development
MH  - Olfactory Pathways/anatomy & histology/*growth & development
MH  - Olfactory Receptor Neurons/anatomy & histology/*growth & development
MH  - Xenopus laevis/anatomy & histology/*growth & development
EDAT- 2005/07/19 09:00
MHDA- 2005/09/28 09:00
CRDT- 2005/07/19 09:00
PHST- 2005/07/19 09:00 [pubmed]
PHST- 2005/09/28 09:00 [medline]
PHST- 2005/07/19 09:00 [entrez]
AID - 10.1002/cne.20655 [doi]
PST - ppublish
SO  - J Comp Neurol. 2005 Sep 5;489(4):403-24. doi: 10.1002/cne.20655.