PMID- 16028412
OWN - NLM
STAT- MEDLINE
DCOM- 20060615
LR  - 20190902
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 293
DP  - 2005
TI  - Microdissection for detecting genetic aberrations in early and advanced human 
      urinary bladder cancer.
PG  - 79-92
AB  - Laser microdissection is an essential method for the investigation of the 
      multistep carcinogenic process in the urinary bladder. Reliable detection of 
      tumor-specific alterations which can be compromised by the presence of normal 
      cells, requires microdissection of pure tumor cell populations (>80%) to detect 
      loss of heterozygosity (LOH) by either fluorescence in situ hybridization (FISH) 
      or sequence analysis. Multiple molecular methods need to be performed in the 
      course of studying often-small lesions. This chapter describes in detail the use 
      of laser microdissection, whole-genome amplification by improved primer extension 
      preamplification (I-PEP)-polymerase chain reaction, and subsequent LOH, FISH, and 
      sequencing analysis in the investigation of urothelial tumors and their precursor 
      lesions. The combination of the described methods allows a wide spectrum of 
      molecular investigations of tumor cells and helps to understand the fundamental 
      alterations involved in urothelial carcinogenesis.
FAU - Hartmann, Arndt
AU  - Hartmann A
AD  - Institute of Pathology, University of Basel, Switzerland.
FAU - Stoehr, Robert
AU  - Stoehr R
FAU - Wild, Peter J
AU  - Wild PJ
FAU - Dietmaier, Wolfgang
AU  - Dietmaier W
FAU - Knuechel, Ruth
AU  - Knuechel R
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Chromosome Aberrations
MH  - Disease Progression
MH  - Early Diagnosis
MH  - Humans
MH  - *Lasers
MH  - Microdissection/*methods
MH  - Urinary Bladder Neoplasms/diagnosis/*genetics/*pathology
RF  - 8
EDAT- 2005/07/21 09:00
MHDA- 2006/06/16 09:00
CRDT- 2005/07/21 09:00
PHST- 2005/07/21 09:00 [pubmed]
PHST- 2006/06/16 09:00 [medline]
PHST- 2005/07/21 09:00 [entrez]
AID - 10.1385/1-59259-853-6:079 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2005;293:79-92. doi: 10.1385/1-59259-853-6:079.