PMID- 16029429
OWN - NLM
STAT- MEDLINE
DCOM- 20050919
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 66
IP  - 2
DP  - 2005 Aug
TI  - HLA-DQB1 sequencing-based typing using newly identified conserved nucleotide 
      sequences in introns 1 and 2.
PG  - 99-106
AB  - Sequencing-based typing (SBT) human leukocyte antigen (HLA) class I and II genes 
      should examine entire exon sequences where polymorphisms lie. Primers for the 
      amplification of complete exons therefore anneal in introns and their design 
      relies on accurate intron sequences being available. We decided to develop a SBT 
      method for HLA-DQB1 using amplification primers which anneal in introns 1 and 2, 
      yet the amount of intron sequence data previously available in databases was 
      sparse. Therefore, we undertook a systematic sequencing of introns 1 and 2 using 
      DNA from cell lines homozygous for DQB1. This study confirmed an earlier report 
      that the non-coding regions of this gene are the most polymorphic seen in the 
      human genome. Intron sequences within an allele group were largely identical, the 
      exceptions being DQB1*0301 differing from other DQB1*03 allele groups and 
      DQB1*0601 differing from all other DQB1*06 alleles. A retroviral Alu element, 
      related to the AluYa5a2 subfamily, was identified uniquely inserted in intron 2 
      of DQB1*02 alleles. For the typing approach, six amplification primers were 
      designed based on conserved allele group sequences covering all of the HLA DQB 
      antigens, and two sequencing primers were also designed which anneal in intron 2. 
      This method has proved to be very robust and has been used as part of a referral 
      DNA sequencing service for a number of years.
FAU - Dunn, P P J
AU  - Dunn PP
AD  - DNA Reference Laboratory, National Blood Service, Southmead Road, Bristol BS10 
      5ND, UK. paul.dunn@nzblood. co.nz
FAU - Day, S
AU  - Day S
FAU - Williams, S
AU  - Williams S
FAU - Bendukidze, N
AU  - Bendukidze N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
SB  - IM
MH  - Alleles
MH  - *Base Sequence
MH  - *Conserved Sequence
MH  - Genetic Variation
MH  - HLA-DQ Antigens/classification/*genetics
MH  - HLA-DQ beta-Chains
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Introns
MH  - Molecular Sequence Data
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2005/07/21 09:00
MHDA- 2005/09/20 09:00
CRDT- 2005/07/21 09:00
PHST- 2005/07/21 09:00 [pubmed]
PHST- 2005/09/20 09:00 [medline]
PHST- 2005/07/21 09:00 [entrez]
AID - TAN445 [pii]
AID - 10.1111/j.1399-0039.2005.00445.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2005 Aug;66(2):99-106. doi: 10.1111/j.1399-0039.2005.00445.x.