PMID- 16029441 OWN - NLM STAT- MEDLINE DCOM- 20050919 LR - 20111117 IS - 0001-2815 (Print) IS - 0001-2815 (Linking) VI - 66 IP - 2 DP - 2005 Aug TI - Identification and characterization of a novel HLA-DRB1 allele, DRB1*0830*. PG - 160-2 AB - Here, we report on the identification and characterization of a novel human leukocyte antigen (HLA) DRB1 allele, DRB1*0830. This allele was identified in a candidate for hematopoietic stem cell donation. While the DNA sequence of HLA-DRB1*0830 most closely matches to DRB1*080202, its amino acid sequence resembles DRB1*080202 and DRB1*0813. Due to a substitution at nucleotide position 286 HLA-typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. Allele-specific DNA sequencing confirmed a 286 T-->A substitution resulting in Phe67Ile. FAU - Hoppe, B AU - Hoppe B AD - Institute of Transfusion Medicine, Campus Virchow-Klinikum, Charite-Universitatsmedizin Berlin, Berlin, Germany. berthold.hoppe@charite.de FAU - Heymann, G A AU - Heymann GA FAU - Kiesewetter, H AU - Kiesewetter H FAU - Salama, A AU - Salama A LA - eng SI - GENBANK/AJ850053 PT - Journal Article PL - England TA - Tissue Antigens JT - Tissue antigens JID - 0331072 RN - 0 (HLA-DR Antigens) RN - 0 (HLA-DRB1 Chains) SB - IM MH - Alleles MH - Base Sequence MH - HLA-DR Antigens/*genetics MH - HLA-DRB1 Chains MH - Histocompatibility Testing MH - Humans MH - Models, Molecular MH - Molecular Sequence Data MH - Nucleic Acid Hybridization MH - Protein Structure, Tertiary EDAT- 2005/07/21 09:00 MHDA- 2005/09/20 09:00 CRDT- 2005/07/21 09:00 PHST- 2005/07/21 09:00 [pubmed] PHST- 2005/09/20 09:00 [medline] PHST- 2005/07/21 09:00 [entrez] AID - TAN431 [pii] AID - 10.1111/j.1399-0039.2005.00431.x [doi] PST - ppublish SO - Tissue Antigens. 2005 Aug;66(2):160-2. doi: 10.1111/j.1399-0039.2005.00431.x.