PMID- 16029868
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20131121
IS  - 1473-0502 (Print)
IS  - 1473-0502 (Linking)
VI  - 33
IP  - 1
DP  - 2005 Aug
TI  - The influence of methylene blue light treatment and methylene blue removal filter 
      on fibrinogen activity states and fibrin polymerisation indices.
PG  - 63-9
AB  - BACKGROUND/OBJECTIVE: Methylene blue light treatment (MBLT) is efficient in 
      inactivating viruses in plasma. However, it may cause alterations in clotting 
      factors, especially fibrinogen (Fg). The true mechanism of such a selective 
      alteration is poorly understood, The effects of MBLT and MB removal filters 
      (MBRF) on Fg concentration, functional activity and fibrin polymerisation were 
      studied. DESIGN: Apheresis plasma collected by Haemonetic MCS Plus (n=10) was 
      split into two equal aliquots. Both were leukofiltered and virally inactivated in 
      a standardized fashion, using Theraflex MB-Plasma Photodynamic viral inactivation 
      Macopharma method. One of the pair was subsequently processed, using Blueflex 
      MBRF to remove residual MB and its by-products. Control plasma samples were 
      obtained after filtration but before MBLT. Samples were also obtained after MBLT 
      and MBRF. All samples were analyzed for the Fg activity, Fg antigen, thrombin 
      clotting time (TT), and alterations fibrin polymerisation indices. RESULTS: After 
      MBLT, mean Fg concentration increased slightly (2%) whereas functional activity 
      decreased by 31%, as compared to control samples. Mean TT prolonged by 6s after 
      MBLT, with no further changes after MBRF. A similar trend was observed using RT. 
      Both thrombin and reptilase triggered fibrin polymerisation were delayed and the 
      polymerisation curves slopes were decreased slightly, with concomitant changes in 
      fibrin opacity in samples from MBLT and MBRF as compared to control. Comparison 
      is made for the first time with a similar changes observed in dysfibrinogenemia. 
      CONCLUSION: MBLT resulted in about 30% decrease in Fg activity but a slight 
      modification in fibrin polymerisation indices, with no additional alteration 
      subsequent to MBRF. These in vitro changes are similar to those seen in plasma 
      from patients with dysfibrinogenemia, which are usually without clinical 
      significance.
FAU - Depasse, Francois
AU  - Depasse F
AD  - LCL, 78, Avenue de Verdun, 94200 Ivry sur Seine, France. f.depasse@wanadoo.fr
FAU - Sensebe, Luc
AU  - Sensebe L
FAU - Seghatchian, Jerard
AU  - Seghatchian J
FAU - Andreu, Georges
AU  - Andreu G
FAU - Samama, Meyer-Michel
AU  - Samama MM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Transfus Apher Sci
JT  - Transfusion and apheresis science : official journal of the World Apheresis 
      Association : official journal of the European Society for Haemapheresis
JID - 101095653
RN  - 0 (Enzyme Inhibitors)
RN  - 9001-31-4 (Fibrin)
RN  - 9001-32-5 (Fibrinogen)
RN  - T42P99266K (Methylene Blue)
MH  - Blood Preservation/methods
MH  - Enzyme Inhibitors/*pharmacology
MH  - Fibrin/*analysis
MH  - Fibrinogen/*analysis
MH  - Humans
MH  - *Light
MH  - Methylene Blue/*pharmacology
MH  - Plasma/*chemistry/virology
MH  - Virus Inactivation/drug effects/radiation effects
EDAT- 2005/07/21 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/07/21 09:00
PHST- 2004/12/09 00:00 [received]
PHST- 2005/02/14 00:00 [accepted]
PHST- 2005/07/21 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/07/21 09:00 [entrez]
AID - S1473-0502(05)00063-7 [pii]
AID - 10.1016/j.transci.2005.02.002 [doi]
PST - ppublish
SO  - Transfus Apher Sci. 2005 Aug;33(1):63-9. doi: 10.1016/j.transci.2005.02.002.