PMID- 16030185
OWN - NLM
STAT- MEDLINE
DCOM- 20051107
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 106
IP  - 8
DP  - 2005 Oct 15
TI  - Monocyte-derived dendritic cells activated by bacteria or by bacteria-stimulated 
      epithelial cells are functionally different.
PG  - 2818-26
AB  - Dendritic cells (DCs) are able to open the tight junctions between adjacent 
      epithelial cells (ECs) and to take up both invasive and noninvasive bacteria 
      directly from the intestinal lumen. In this study, we describe a tight cross talk 
      between ECs and human monocyte-derived DCs (MoDCs) in bacterial handling across 
      epithelial monolayers. We show that the release of proinflammatory mediators by 
      ECs in response to bacteria is dependent on bacterial invasiveness and on the 
      presence of flagella. This correlates with the capacity of EC-derived factors to 
      modulate MoDC function. MoDCs incubated with supernatants of bacteria-treated ECs 
      are "noninflammatory" as they release interleukin-10 (IL-10) but not IL-12 and 
      can drive only T helper (Th)-2 type T cells. Moreover, noninflammatory MoDCs 
      release chemokines aimed at recruiting Th2 and T-regulatory cells. In contrast, 
      when MoDCs are incubated with ECs and bacteria in a transwell coculture system, 
      and can contact directly the bacteria across stimulated EC monolayers, they are 
      more inflammatory as they release IL-12 and IL-10 and induce both Th1 and Th2 
      responses. These results suggest that ECs are not simply a barrier to bacteria 
      entering via the oral route, but they actively influence the activating 
      properties of DCs.
FAU - Rimoldi, Monica
AU  - Rimoldi M
AD  - Department of Experimental Oncology, European Institute of Oncology, Istituto di 
      Ricerche Farmacologiche, Mario Negri, Milan, Italy.
FAU - Chieppa, Marcello
AU  - Chieppa M
FAU - Larghi, Paola
AU  - Larghi P
FAU - Vulcano, Marisa
AU  - Vulcano M
FAU - Allavena, Paola
AU  - Allavena P
FAU - Rescigno, Maria
AU  - Rescigno M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050719
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCL20 protein, mouse)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Chemokine CCL20
MH  - Chemokines, CC/metabolism
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Epithelial Cells/cytology/*immunology/metabolism/*microbiology
MH  - Flagella/physiology
MH  - Gram-Positive Bacteria/cytology/pathogenicity/*physiology
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Macrophage Inflammatory Proteins/metabolism
MH  - Mice
MH  - Monocytes/*cytology/immunology/metabolism
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
EDAT- 2005/07/21 09:00
MHDA- 2005/11/08 09:00
CRDT- 2005/07/21 09:00
PHST- 2005/07/21 09:00 [pubmed]
PHST- 2005/11/08 09:00 [medline]
PHST- 2005/07/21 09:00 [entrez]
AID - S0006-4971(20)69125-X [pii]
AID - 10.1182/blood-2004-11-4321 [doi]
PST - ppublish
SO  - Blood. 2005 Oct 15;106(8):2818-26. doi: 10.1182/blood-2004-11-4321. Epub 2005 Jul 
      19.