PMID- 16034191
OWN - NLM
STAT- MEDLINE
DCOM- 20060110
LR  - 20190608
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 51
IP  - 5
DP  - 2005 Oct
TI  - Estrogen inhibits interleukin-18 mRNA expression in the mouse uterus.
PG  - 639-47
AB  - Interleukin-18 (IL-18) is a proinflammatory cytokine expressed in female 
      reproductive organs in humans, rats and mice. The physiological roles of uterine 
      IL-18 and the regulatory mechanisms of IL-18 gene expression are unclear. The 
      present study aimed to clarify the effects of estradiol-17beta (E2) and 
      progesterone (P4) on IL-18 mRNA expression in the mouse uterus. Distribution and 
      expression levels of IL-18 mRNA were studied using an RNase protection assay. 
      Expression of IL-18 mRNA was observed in all organs studied, including testes, 
      ovaries and uteri. The uterine IL-18 mRNA level of estrous mice was higher than 
      that of diestrous mice. E2 treatment (1, 5, 25 or 250 ng/mouse) decreased uterine 
      IL-18 mRNA levels in ovariectomized mice dose-dependently. E2 treatment acutely 
      decreased IL-18 mRNA levels 3 h after injection, but these levels returned to the 
      initial level after 48 h. P4 treatment (1 mg/mouse) decreased uterine IL-18 mRNA 
      levels after 12 h, but levels returned to the initial level after 48 h. Both 
      uterine IL-18 and IL-18Ralpha mRNAs were detected in cultured endometrial 
      epithelial and stromal cells. These results suggest that uterine IL-18 expression 
      is reduced by sex steroid hormones and that IL-18 acts on endometrial cells in a 
      paracrine or autocrine manner.
FAU - Murakami, Yousuke
AU  - Murakami Y
AD  - Department of Biology, Faculty of Science, Okayama University, Japan.
FAU - Otsuki, Mariko
AU  - Otsuki M
FAU - Kusumoto, Kenji
AU  - Kusumoto K
FAU - Takeuchi, Sakae
AU  - Takeuchi S
FAU - Takahashi, Sumio
AU  - Takahashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050721
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Epithelial Cells/physiology
MH  - Estradiol/*pharmacology
MH  - Estrous Cycle/physiology
MH  - Female
MH  - Interleukin-18/*biosynthesis/genetics
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Ovariectomy
MH  - Progesterone/*pharmacology
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - Receptors, Interleukin/biosynthesis/genetics
MH  - Receptors, Interleukin-18
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonucleases/metabolism
MH  - Stromal Cells/physiology
MH  - Uterus/*drug effects/metabolism/physiology
EDAT- 2005/07/22 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/07/22 09:00
PHST- 2005/07/22 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/07/22 09:00 [entrez]
AID - JST.JSTAGE/jrd/17029 [pii]
AID - 10.1262/jrd.17029 [doi]
PST - ppublish
SO  - J Reprod Dev. 2005 Oct;51(5):639-47. doi: 10.1262/jrd.17029. Epub 2005 Jul 21.