PMID- 16036370
OWN - NLM
STAT- MEDLINE
DCOM- 20051011
LR  - 20180816
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 39
IP  - 8
DP  - 2005 Aug
TI  - Effect of vegetables on human phenolsulfotransferases in relation to their 
      antioxidant activity and total phenolics.
PG  - 893-904
AB  - Epidemiology studies have shown that consumption of fruits and vegetables is 
      associated with the prevention of chronic diseases such as cancer and 
      cardiovascular disease. Induction of cellular phase II detoxifying enzymes is 
      associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are 
      traditionally known as phase II drug-metabolizing or detoxifying enzymes that 
      facilitate the removal of drugs and other xenobiotic compounds. Phenolic acids 
      are known to increase the activities of PSTs. In the present study, human HepG2 
      cells were used as model to investigate the influence of twenty vegetables on 
      human PST activity and to evaluate the relationships to their antioxidant 
      activity and total phenolics content. The result showed that PST-P activity was 
      significantly (p < 0.01) induced by asparagus, broccoli, cauliflower, celery and 
      eggplant, whereas PST-M activity was induced by asparagus, broccoli, carrot, 
      eggplant and potato at a concentration of 100 microg/ml. The vegetable extracts 
      that induced both forms of PSTs activities were found to have higher antioxidant 
      capacities and total phenolic content in the oxygen radical absorbance capacity 
      (ORAC) and Folin-Ciocalteu assay. The major polyphenols in broccoli, the most 
      potential inducer in both forms of PSTs activities, was antioxidant phenolic 
      acids. HPLC retention times and standard spiked indicated the presence of gallic 
      acid, p-hydroxybenzoic acid, p-coumaric acid, gentisic acid and ferulic acid in 
      broccoli. The overall effect of vegetables tested on the activity of PST-P was 
      well correlated to their ORAC value and total phenolics content (r= 0.82, p < 
      0.05 and r = 0.78, p < 0.05). These results imply that vegetables have a 
      capability of inducing PST activity, and the PST induction may be possibly 
      ascribed to antioxidant phenolic acids in vegetable extracts.
FAU - Yeh, Chi-Tai
AU  - Yeh CT
AD  - Department of Food Science and Biotechnology, National Chung Hsing University, 
      250 Kuokuang Road, Taichung, 40227, Taiwan.
FAU - Yen, Gow-Chin
AU  - Yen GC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Antioxidants)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
RN  - EC 2.8.2.1 (monoamine-sulfating phenol sulfotransferase)
RN  - I3P9R8317T (phenolic acid)
SB  - IM
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - Arylsulfotransferase
MH  - Brassica/chemistry
MH  - Carcinoma, Hepatocellular
MH  - Cell Line, Tumor
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Hydroxybenzoates/*analysis
MH  - Plant Extracts/isolation & purification/pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reactive Oxygen Species/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sulfotransferases/biosynthesis/genetics/*metabolism
MH  - Vegetables/*chemistry
EDAT- 2005/07/23 09:00
MHDA- 2005/10/12 09:00
CRDT- 2005/07/23 09:00
PHST- 2005/07/23 09:00 [pubmed]
PHST- 2005/10/12 09:00 [medline]
PHST- 2005/07/23 09:00 [entrez]
AID - J7H8LH6044804Q05 [pii]
AID - 10.1080/10715760500150424 [doi]
PST - ppublish
SO  - Free Radic Res. 2005 Aug;39(8):893-904. doi: 10.1080/10715760500150424.