PMID- 16037388
OWN - NLM
STAT- MEDLINE
DCOM- 20051215
LR  - 20220310
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 106
IP  - 10
DP  - 2005 Nov 15
TI  - Induction of HM1.24 peptide-specific cytotoxic T lymphocytes by using 
      peripheral-blood stem-cell harvests in patients with multiple myeloma.
PG  - 3538-45
AB  - HM1.24 antigen is preferentially overexpressed in multiple myeloma (MM) cells but 
      not in normal cells. To explore the potential of HM1.24 as a target for cellular 
      immunotherapy, we selected 4 HM1.24-derived peptides that possess binding motifs 
      for HLA-A2 or HLA-A24 by using 2 computer-based algorithms. The ability of these 
      peptides to generate cytotoxic T lymphocytes (CTLs) was examined in 20 healthy 
      donors and 6 patients with MM by a reverse immunologic approach. Dendritic cells 
      (DCs) were induced from peripheral-blood mononuclear cells of healthy donors or 
      peripheral-blood stem-cell (PBSC) harvests from patients with MM, and autologous 
      CD8(+) T cells were stimulated with HM1.24 peptide-pulsed DCs. Both 
      interferon-gamma-producing and cytotoxic responses were observed after 
      stimulation with either HM1.24-126 or HM1.24-165 peptides in HLA-A2 or HLA-A24 
      individuals. The peptide-specific recognition of these CTLs was further confirmed 
      by tetramer assay and cold target inhibition assay. Importantly, HM1.24-specific 
      CTLs were also induced from PBSC harvests from patients with MM and these CTLs 
      were able to kill MM cells in an HLA-restricted manner. These results indicate 
      the existence of functional DCs and HM1.24-specific CTL precursors within PBSC 
      harvests and provide the basis for cellular immunotherapy in combination with 
      autologous PBSC transplantation in MM.
FAU - Jalili, Ali
AU  - Jalili A
AD  - Department of Medicine and Bioregulatory Sciences, Institute of Health 
      Biosciences, University of Tokushima Graduate School, Japan.
FAU - Ozaki, Shuji
AU  - Ozaki S
FAU - Hara, Tomoko
AU  - Hara T
FAU - Shibata, Hironobu
AU  - Shibata H
FAU - Hashimoto, Toshihiro
AU  - Hashimoto T
FAU - Abe, Masahiro
AU  - Abe M
FAU - Nishioka, Yasuhiko
AU  - Nishioka Y
FAU - Matsumoto, Toshio
AU  - Matsumoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050721
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (BST2 protein, human)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptides)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, CD
MH  - Cell Line, Tumor
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - GPI-Linked Proteins
MH  - HLA-A Antigens/immunology
MH  - HLA-A2 Antigen/immunology
MH  - HLA-A24 Antigen
MH  - Hematopoietic Stem Cells/cytology/*immunology
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Male
MH  - Membrane Glycoproteins/*immunology/pharmacology
MH  - Multiple Myeloma/*immunology/therapy
MH  - Peptides/*immunology/pharmacology
MH  - Peripheral Blood Stem Cell Transplantation/methods
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transplantation, Homologous
EDAT- 2005/07/23 09:00
MHDA- 2005/12/16 09:00
CRDT- 2005/07/23 09:00
PHST- 2005/07/23 09:00 [pubmed]
PHST- 2005/12/16 09:00 [medline]
PHST- 2005/07/23 09:00 [entrez]
AID - S0006-4971(20)68499-3 [pii]
AID - 10.1182/blood-2005-04-1438 [doi]
PST - ppublish
SO  - Blood. 2005 Nov 15;106(10):3538-45. doi: 10.1182/blood-2005-04-1438. Epub 2005 
      Jul 21.