PMID- 16037408
OWN - NLM
STAT- MEDLINE
DCOM- 20060110
LR  - 20220309
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 78
IP  - 4
DP  - 2005 Oct
TI  - A novel role for Notch ligand Delta-1 as a regulator of human Langerhans cell 
      development from blood monocytes.
PG  - 921-9
AB  - Human Langerhans cells (LCs) are of hematopoietic origin, but cytokine regulation 
      of their development is not fully understood. Notch ligand Delta-1 is expressed 
      in a proportion of the skin. Granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) and transforming growth factor-beta1 (TGF-beta1) are also secreted in 
      the skin. We report here that Delta-1, in concert with GM-CSF and TGF-beta1, 
      induces the differentiation of human CD14(+) blood monocytes into cells that 
      express LC markers: CD1a, Langerin, cutaneous lymphocyte-associated antigen, CC 
      chemokine receptor 6, E-cadherin, and Birbeck granules. The resulting cells 
      display phagocytic activity and chemotaxis to macrophage inflammatory 
      protein-1alpha (MIP-1alpha). In response to CD40 ligand and tumor necrosis factor 
      alpha, the cells acquire a mature phenotype of dendritic cells that is 
      characterized by up-regulation of human leukocyte antigen (HLA)-ABC, HLA-DR, 
      CD80, CD86, CD40, and CD54 and appearance of CD83. These cells in turn show 
      chemotaxis toward MIP-1beta and elicit activation of CD8(+) T cells and T helper 
      cell type 1 polarization of CD4(+) T cells. Thus, blood monocytes can give rise 
      to LCs upon exposure to the skin cytokine environment consisting of Delta-1, 
      GM-CSF, and TGF-beta1, which may be, in part, relevant to the development of 
      human epidermal LCs. Our results extend the functional scope of Notch ligand 
      delta-1 in human hematopoiesis.
FAU - Hoshino, Natsuki
AU  - Hoshino N
AD  - Department of Hematology and Oncology, Mie University School of Medicine, Mie 
      514-8507, Japan. n-kata@clin.medic.mie-u.ac.jp
FAU - Katayama, Naoyuki
AU  - Katayama N
FAU - Shibasaki, Tetsunori
AU  - Shibasaki T
FAU - Ohishi, Kohshi
AU  - Ohishi K
FAU - Nishioka, Junji
AU  - Nishioka J
FAU - Masuya, Masahiro
AU  - Masuya M
FAU - Miyahara, Yoshihiro
AU  - Miyahara Y
FAU - Hayashida, Masahiko
AU  - Hayashida M
FAU - Shimomura, Daiki
AU  - Shimomura D
FAU - Kato, Takuma
AU  - Kato T
FAU - Nakatani, Kaname
AU  - Nakatani K
FAU - Nishii, Kazuhiro
AU  - Nishii K
FAU - Kuribayashi, Kagemasa
AU  - Kuribayashi K
FAU - Nobori, Tsutomu
AU  - Nobori T
FAU - Shiku, Hiroshi
AU  - Shiku H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050721
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Membrane Proteins)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transcription Factor HES-1)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (delta protein)
RN  - 149348-15-2 (HES1 protein, human)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.46 (BACE1 protein, human)
SB  - IM
MH  - Amyloid Precursor Protein Secretases
MH  - Aspartic Acid Endopeptidases
MH  - Basic Helix-Loop-Helix Transcription Factors/drug effects/genetics
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Polarity/physiology
MH  - Cells, Cultured
MH  - Chemotaxis/physiology
MH  - Endopeptidases/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism/pharmacology
MH  - Homeodomain Proteins/drug effects/genetics
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Langerhans Cells/*cytology/drug effects/*physiology
MH  - Ligands
MH  - Lipopolysaccharide Receptors/*blood/drug effects/metabolism
MH  - Membrane Proteins/pharmacology/*physiology
MH  - Monocytes/*cytology/drug effects/*physiology
MH  - Phenotype
MH  - Transcription Factor HES-1
MH  - Transforming Growth Factor beta/metabolism/pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2005/07/23 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/07/23 09:00
PHST- 2005/07/23 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/07/23 09:00 [entrez]
AID - jlb.1204746 [pii]
AID - 10.1189/jlb.1204746 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2005 Oct;78(4):921-9. doi: 10.1189/jlb.1204746. Epub 2005 Jul 21.