PMID- 16038636
OWN - NLM
STAT- MEDLINE
DCOM- 20060503
LR  - 20181201
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 26
IP  - 8
DP  - 2005 Aug
TI  - Effect of curcumin on multidrug resistance in resistant human gastric carcinoma 
      cell line SGC7901/VCR.
PG  - 1009-16
AB  - AIM: To investigate the reversal effects of curcumin on multidrug resistance 
      (MDR) in a resistant human gastric carcinoma cell line. METHODS: The cytotoxic 
      effect of vincristine (VCR) was evaluated by MTT assay. The cell apoptosis 
      induced by VCR was determined by propidium iodide (PI)-stained flow cytometry 
      (FCM) and a morphological assay using acridine orange (AO)/ethidium bromide (EB) 
      dual staining. P-glycoprotein (P-gp) function was demonstrated by the 
      accumulation and efflux of rhodamine123 (Rh123) using FCM. The expression of P-gp 
      and the activation of caspase-3 were measured by FCM using fluorescein 
      isothiocyanate (FITC)-conjugated anti-P-gp and anti-cleaved caspase-3 antibodies, 
      respectively. RESULTS: Curcumin, at concentrations of 5 micromol/L, 10 
      micromol/L, or 20 micromol/L, had no cytotoxic effect on a parent human gastric 
      carcinoma cell line (SGC7901) or its VCR-resistant variant cell line 
      (SGC7901/VCR). The VCR-IC50 value of the SGC7901/VCR cells was 45 times more than 
      that of the SGC7901cells and the SGC7901/VCR cells showed apoptotic resistance to 
      VCR. SGC7901/VCR cells treated with 5 micromol/L, 10 micromol/L, or 20 micromol/L 
      curcumin decreased the IC50 value of VCR and promoted VCR-mediated apoptosis in a 
      dose-dependent manner. Curcumin (10 micromol/L) increased Rh123 accumulation and 
      inhibited the efflux of Rh123 in SGC7901/VCR cells, but did not change the 
      accumulation and efflux of Rh123 in SGC7901 cells. P-gp was overexpressed in 
      SGC7901/VCR cells, whereas it was downregulated after a 24-h treatment with 
      curcumin (10 micromol/L). Resistant cells treated with 1 mumol/L VCR alone showed 
      77% lower levels of caspase-3 activation relative to SGC7901 cells, but the 
      activation of caspase-3 in the resistant cell line increased by 44% when cells 
      were treated with VCR in combination with curcumin. CONCLUSION: Curcumin can 
      reverse the MDR of the human gastric carcinoma SGC7901/VCR cell line. This might 
      be associated with decreased P-gp function and expression, and the promotion of 
      caspase-3 activation in MDR cells.
FAU - Tang, Xiao-qing
AU  - Tang XQ
AD  - Department of Physiology, Nanhua University, Hengyang 421001, China.
FAU - Bi, Hu
AU  - Bi H
FAU - Feng, Jian-qiang
AU  - Feng JQ
FAU - Cao, Jian-guo
AU  - Cao JG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Antineoplastic Agents)
RN  - 1N3CZ14C5O (Rhodamine 123)
RN  - 5J49Q6B70F (Vincristine)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/biosynthesis/physiology
MH  - Antineoplastic Agents/pharmacology
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Curcumin/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Resistance, Multiple/*drug effects
MH  - Drug Resistance, Neoplasm/drug effects
MH  - Enzyme Activation/drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Rhodamine 123/pharmacokinetics
MH  - Stomach Neoplasms/metabolism/pathology
MH  - Vincristine/*pharmacology
EDAT- 2005/07/26 09:00
MHDA- 2006/05/04 09:00
CRDT- 2005/07/26 09:00
PHST- 2005/07/26 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2005/07/26 09:00 [entrez]
AID - 10.1111/j.1745-7254.2005.00149.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2005 Aug;26(8):1009-16. doi: 
      10.1111/j.1745-7254.2005.00149.x.