PMID- 16039684
OWN - NLM
STAT- MEDLINE
DCOM- 20060501
LR  - 20201209
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 212
IP  - 1
DP  - 2006 Apr 1
TI  - The cytokine-dependent MUTZ-3 cell line as an in vitro model for the screening of 
      contact sensitizers.
PG  - 14-23
AB  - Langerhans cells (LC) are key mediators of contact allergenicity in the skin. 
      However, no in vitro methods exist which are based on the activation process of 
      LC to predict the sensitization potential of chemicals. In this study, we have 
      evaluated the performances of MUTZ-3, a cytokine-dependent human monocytic cell 
      line, in its response to sensitizers. First, we compared undifferentiated MUTZ-3 
      cells with several standard human cells such as THP-1, KG-1, HL-60, K-562, and 
      U-937 in their response to the strong sensitizer DNCB and the irritant SDS by 
      monitoring the expression levels of HLA-DR, CD54, and CD86 by flow cytometry. 
      Only MUTZ-3 and THP-1 cells show a strong and specific response to sensitizer, 
      while other cell lines showed very variable responses. Then, we tested MUTZ-3 
      cells against a wider panel of sensitizers and irritants on a broader spectrum of 
      cell surface markers (HLA-DR, CD40, CD54, CD80, CD86, B7-H1, B7-H2, B7-DC). Of 
      these markers, CD86 proved to be the most reliable since it detected all 
      sensitizers, including benzocaine, a classical false negative in local lymph node 
      assay (LLNA) but not irritants. We confirmed the MUTZ-3 response to DNCB by 
      real-time PCR analysis. Taken together, our data suggest that undifferentiated 
      MUTZ-3 cells may represent a valuable in vitro model for the screening of 
      potential sensitizers.
FAU - Azam, Philippe
AU  - Azam P
AD  - AFSSAPS, Unite BCM, 635 Rue de la Garenne, 34740 Vendargues, France.
FAU - Peiffer, Jean-Luc
AU  - Peiffer JL
FAU - Chamousset, Delphine
AU  - Chamousset D
FAU - Tissier, Marie-Helene
AU  - Tissier MH
FAU - Bonnet, Pierre-Antoine
AU  - Bonnet PA
FAU - Vian, Laurence
AU  - Vian L
FAU - Fabre, Isabelle
AU  - Fabre I
FAU - Ourlin, Jean-Claude
AU  - Ourlin JC
LA  - eng
PT  - Journal Article
DEP - 20050721
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Antigens, Surface)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Inducible T-Cell Co-Stimulator Ligand)
RN  - 0 (Irritants)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Antigens, Surface/biosynthesis
MH  - B7-1 Antigen/pharmacology
MH  - B7-2 Antigen/genetics
MH  - CD40 Antigens/biosynthesis
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Tumor
MH  - Culture Media
MH  - Cytokines/*physiology
MH  - Dermatitis, Contact/*physiopathology
MH  - Dinitrochlorobenzene/pharmacology
MH  - Drug Evaluation, Preclinical
MH  - Flow Cytometry
MH  - HLA-DR Antigens/genetics
MH  - Humans
MH  - Inducible T-Cell Co-Stimulator Ligand
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Irritants/pharmacology/*toxicity
MH  - Male
MH  - Predictive Value of Tests
MH  - RNA/biosynthesis/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Dodecyl Sulfate/pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2005/07/26 09:00
MHDA- 2006/05/02 09:00
CRDT- 2005/07/26 09:00
PHST- 2005/03/01 00:00 [received]
PHST- 2005/06/01 00:00 [revised]
PHST- 2005/06/14 00:00 [accepted]
PHST- 2005/07/26 09:00 [pubmed]
PHST- 2006/05/02 09:00 [medline]
PHST- 2005/07/26 09:00 [entrez]
AID - S0041-008X(05)00383-2 [pii]
AID - 10.1016/j.taap.2005.06.018 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2006 Apr 1;212(1):14-23. doi: 10.1016/j.taap.2005.06.018. 
      Epub 2005 Jul 21.