PMID- 16040397
OWN - NLM
STAT- MEDLINE
DCOM- 20051128
LR  - 20220311
IS  - 1465-3249 (Print)
IS  - 1465-3249 (Linking)
VI  - 7
IP  - 2
DP  - 2005
TI  - Methods for isolating circulating epithelial cells and criteria for their 
      classification as carcinoma cells.
PG  - 171-85
AB  - Novel assay methods developed for the isolation and characterization of 
      circulating tumor cells (CTC) of epithelial origin offer the potential of markers 
      for the non-invasive gathering of clinical information relevant to the diagnosis, 
      evolution and treatment of carcinoma. Of the numerous techniques currently used 
      to analyze CTC, slide-based assays are perhaps the most common. While traditional 
      combined immunocytochemical/brightfield microscopy systems continue to be the 
      most frequently employed, fluorescence-based analysis is gaining in importance. 
      This is partly because fluorescence microscopy analysis of slide-mounted CTC can 
      provide simultaneously cytogenetic as well as morphologic and multiple phenotypic 
      information. In particular, fluorescence microscopy analysis of slide-mounted CTC 
      can accurately determine genetic changes at the chromosomal level in patients 
      with recurrent disease. More importantly, by identifying genetic aberrations in 
      CTC, it becomes possible to choose those patients most likely to benefit from a 
      given treatment. The potential of this technique has already been demonstrated by 
      employing fluorescence in situ hybridization (FISH) methods to measure expression 
      of the HER2/neu gene in tissue from patients with breast carcinoma for the 
      specific purpose of identifying those patients most likely to respond to 
      Trastuzumab targeted therapy. Here, we review the major methodologies used in the 
      preparation and analysis of the slide-based assays.
FAU - Fehm, T
AU  - Fehm T
AD  - Department of Obstetrics and Gynecology, University of Tuebingen, Calver Street 
      7, 72076 Tuebingen, Germany.
FAU - Solomayer, E F
AU  - Solomayer EF
FAU - Meng, S
AU  - Meng S
FAU - Tucker, T
AU  - Tucker T
FAU - Lane, N
AU  - Lane N
FAU - Wang, J
AU  - Wang J
FAU - Gebauer, G
AU  - Gebauer G
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
SB  - IM
MH  - Carcinoma/diagnosis/genetics/*pathology
MH  - Cell Separation/methods
MH  - *Chromosomes, Human/genetics
MH  - Epithelial Cells/metabolism/*pathology
MH  - Humans
MH  - Karyotyping
RF  - 62
EDAT- 2005/07/26 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/07/26 09:00
PHST- 2005/07/26 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/07/26 09:00 [entrez]
AID - NR4542451H895183 [pii]
AID - 10.1080/14653240510027082 [doi]
PST - ppublish
SO  - Cytotherapy. 2005;7(2):171-85. doi: 10.1080/14653240510027082.