PMID- 16044287
OWN - NLM
STAT- MEDLINE
DCOM- 20060103
LR  - 20131121
IS  - 1615-7591 (Print)
IS  - 1615-7591 (Linking)
VI  - 28
IP  - 1
DP  - 2005 Nov
TI  - Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter 
      oxydans in a semi-continuous two-stage repeated-fed-batch process.
PG  - 37-43
AB  - The influence of the product inhibition by dihydroxyacetone (DHA) on 
      Gluconobacter oxydans for a novel semi-continuous two-stage repeated-fed-batch 
      process was examined quantitatively. It was shown that the culture was able to 
      grow up to a DHA concentration of 80 kg m(-3) without any influence of product 
      inhibition. The regeneration capability of the reversibly product inhibited 
      culture from a laboratory-scale bioreactor system was observed up to a DHA 
      concentration of about 160 kg m(-3). At higher DHA concentrations, the culture 
      was irreversibly product inhibited. However, due to the robust membrane-bound 
      glycerol dehydrogenase of G. oxydans, product formation was still active for a 
      prolonged period of time. The reachable maximum final DHA concentration was as 
      high as 220 kg m(-3). The lag phases for growth increased exponentially with 
      increasing DHA threshold values of the first reactor stage. These results 
      correlated well with fluorescence in situ hybridization (FISH) measurements 
      confirming that the number of active cells decreased exponentially with 
      increasing DHA concentrations.
FAU - Bauer, R
AU  - Bauer R
AD  - Institute of Chemical Engineering, Munich University of Technology, 
      Boltzmannstrasse 15, 85747 Garching, Germany.
FAU - Katsikis, N
AU  - Katsikis N
FAU - Varga, S
AU  - Varga S
FAU - Hekmat, D
AU  - Hekmat D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051028
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - O10DDW6JOO (Dihydroxyacetone)
SB  - IM
MH  - Bioreactors/*microbiology
MH  - Cell Culture Techniques/instrumentation/*methods
MH  - Cell Proliferation
MH  - Dihydroxyacetone/*metabolism
MH  - Gluconobacter oxydans/*growth & development/*metabolism
EDAT- 2005/07/27 09:00
MHDA- 2006/01/04 09:00
CRDT- 2005/07/27 09:00
PHST- 2005/03/21 00:00 [received]
PHST- 2005/06/03 00:00 [accepted]
PHST- 2005/07/27 09:00 [pubmed]
PHST- 2006/01/04 09:00 [medline]
PHST- 2005/07/27 09:00 [entrez]
AID - 10.1007/s00449-005-0009-0 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2005 Nov;28(1):37-43. doi: 10.1007/s00449-005-0009-0. 
      Epub 2005 Oct 28.