PMID- 16045989
OWN - NLM
STAT- MEDLINE
DCOM- 20060329
LR  - 20071115
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 43
IP  - 7
DP  - 2006 Mar
TI  - Gene expression profiling of the effects of intravenous immunoglobulin in human 
      whole blood.
PG  - 939-49
AB  - Intravenous immunoglobulin (IVIG) is involved in many complex mechanisms that act 
      in synergy including expression and function of Fc receptors, complement 
      activation, the cytokine network, interaction with the anti-idiotypic network and 
      modulation of B and T cell activation. To gain insight into the early effects of 
      IVIG on this broad range of activities at the gene level we performed DNA 
      microarray analysis. Human whole blood was incubated in vitro for 4 h followed by 
      extraction of RNA which was hybridized to a chip containing 8793 genes. About 75 
      upregulated genes and 21 downregulated genes were identified using a cut off for 
      the false discovery rate of 5%. These genes are associated with a wide range of 
      cellular immune functions in line with the broad mechanism of action of IVIG. A 
      striking upregulation of a series of genes coding for chemokines was measured. 
      This finding was confirmed at the protein level as pharmacologically relevant 
      concentrations of CXCL9 and CXCL10 were measured in serum. Interestingly, IVIG 
      shows a partial overlap of its gene expression program with lipopolysaccharide. 
      Our data suggests multiple hypotheses regarding the pharmacology of IVIG that 
      must be validated by complementary studies.
FAU - Ghielmetti, Marco
AU  - Ghielmetti M
AD  - ZLB Behring AG, Wankdorfstrasse 10, 3000 Bern 22, Switzerland. 
      marco.ghielmetti@dkf6.unibe.ch
FAU - Bellis, Michel
AU  - Bellis M
FAU - Spycher, Martin O
AU  - Spycher MO
FAU - Miescher, Sylvia
AU  - Miescher S
FAU - Vergeres, Guy
AU  - Vergeres G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050719
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Blood/*drug effects/immunology
MH  - Chemokine CXCL10
MH  - Chemokine CXCL9
MH  - Chemokines, CXC/blood/genetics
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Genes/drug effects
MH  - Humans
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Intercellular Signaling Peptides and Proteins/blood/genetics
MH  - Lipopolysaccharides/pharmacology
EDAT- 2005/07/28 09:00
MHDA- 2006/03/30 09:00
CRDT- 2005/07/28 09:00
PHST- 2005/03/29 00:00 [received]
PHST- 2005/07/28 09:00 [pubmed]
PHST- 2006/03/30 09:00 [medline]
PHST- 2005/07/28 09:00 [entrez]
AID - S0161-5890(05)00212-9 [pii]
AID - 10.1016/j.molimm.2005.06.024 [doi]
PST - ppublish
SO  - Mol Immunol. 2006 Mar;43(7):939-49. doi: 10.1016/j.molimm.2005.06.024. Epub 2005 
      Jul 19.