PMID- 16046163
OWN - NLM
STAT- MEDLINE
DCOM- 20051020
LR  - 20240306
IS  - 1096-6374 (Print)
IS  - 1096-6374 (Linking)
VI  - 15
IP  - 4
DP  - 2005 Aug
TI  - Maternal nutrient restriction during early to mid gestation up-regulates cardiac 
      insulin-like growth factor (IGF) receptors associated with enlarged ventricular 
      size in fetal sheep.
PG  - 291-9
AB  - Intrauterine undernutrition is associated with a high incidence of cardiovascular 
      diseases in adulthood. We previously showed that maternal nutrient restriction 
      during early to mid gestation produces ventricular enlargement, although the 
      mechanism is unknown. We examined myocardial expression of insulin-like growth 
      factor I (IGF-1), IGF-2, IGF binding protein 3 (IGFBP-3), IGF-receptor 1 (IGF-1R) 
      and IGF-2R in fetal sheep with maternal undernutrition. Multiparous ewes were fed 
      with 50% (nutrient-restricted, NR) or 100% (control-fed, C) of NRC requirements 
      from day 28 to 78 of gestation. Some of NR and C ewes were euthanized on day 78, 
      and the rest were fed 100% NRC requirements from day 79 to 135 of gestation. At 
      necropsy on day 78 or day 135 of gestation, gravid uteri were recovered. mRNA 
      expression of IGF-1 and IGF-2 in ventricles were measured with RT-PCR, and 
      protein expression of IGF-1R, IGF-2R, IGFBP-3 was quantitated with Western blot. 
      Crown-rump length was reduced and left ventricle was enlarged in NR fetuses on 
      day 78. At day 135 after re-alimentation, ventricular weights were similar 
      between the two groups although ventricular wall thicknesses were greater in NR 
      than C fetuses. No difference was found in IGF-1, IGF-2 or IGFBP-3 levels between 
      the NR and C groups at either gestational age. Protein expression of IGF-1R and 
      IGF-2R in the left ventricle and IGF-1R in the right ventricle was significantly 
      elevated in the NR group on day 78 of gestation. Only IGF-1R expression remained 
      elevated after late gestational re-alimentation in association with increases in 
      ventricular wall thickness. Our study suggest that maternal undernutrition from 
      early to mid gestation may change the expression of IGF-1R and IGF-2R in fetal 
      myocardium, and play a role in cardiac ventricular enlargement in fetal sheep.
FAU - Dong, Feng
AU  - Dong F
AD  - Division of Pharmaceutical Sciences and Center for Cardiovascular Research and 
      Alternative Medicine, University of Wyoming, Laramie, WY 82071-3375, USA.
FAU - Ford, Stephen P
AU  - Ford SP
FAU - Fang, Cindy X
AU  - Fang CX
FAU - Nijland, Mark J
AU  - Nijland MJ
FAU - Nathanielsz, Peter W
AU  - Nathanielsz PW
FAU - Ren, Jun
AU  - Ren J
LA  - eng
GR  - 1R03 AG 21324-01/AG/NIA NIH HHS/United States
GR  - P01 HD021350/HD/NICHD NIH HHS/United States
GR  - P20 RR 16474/RR/NCRR NIH HHS/United States
GR  - P20 RR 15640/RR/NCRR NIH HHS/United States
GR  - HD 21350/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Scotland
TA  - Growth Horm IGF Res
JT  - Growth hormone & IGF research : official journal of the Growth Hormone Research 
      Society and the International IGF Research Society
JID - 9814320
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, IGF Type 2)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - *Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Female
MH  - Fetus/chemistry
MH  - Gestational Age
MH  - Heart Ventricles/*metabolism
MH  - Insulin-Like Growth Factor Binding Protein 3/*metabolism
MH  - Insulin-Like Growth Factor I/genetics
MH  - Insulin-Like Growth Factor II/genetics
MH  - Pregnancy
MH  - Pregnancy, Animal
MH  - RNA, Messenger/analysis
MH  - Receptor, IGF Type 1/*metabolism
MH  - Receptor, IGF Type 2/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sheep/*metabolism
EDAT- 2005/07/28 09:00
MHDA- 2005/10/21 09:00
CRDT- 2005/07/28 09:00
PHST- 2005/05/01 00:00 [received]
PHST- 2005/05/26 00:00 [revised]
PHST- 2005/05/28 00:00 [accepted]
PHST- 2005/07/28 09:00 [pubmed]
PHST- 2005/10/21 09:00 [medline]
PHST- 2005/07/28 09:00 [entrez]
AID - S1096-6374(05)00058-4 [pii]
AID - 10.1016/j.ghir.2005.05.003 [doi]
PST - ppublish
SO  - Growth Horm IGF Res. 2005 Aug;15(4):291-9. doi: 10.1016/j.ghir.2005.05.003.