PMID- 16050900
OWN - NLM
STAT- MEDLINE
DCOM- 20051114
LR  - 20220408
IS  - 0021-8782 (Print)
IS  - 1469-7580 (Electronic)
IS  - 0021-8782 (Linking)
VI  - 207
IP  - 2
DP  - 2005 Aug
TI  - Development of sensory innervation in rat tibia: co-localization of CGRP and 
      substance P with growth-associated protein 43 (GAP-43).
PG  - 135-44
AB  - The development of sensory innervation in long bones was investigated in rat 
      tibia in fetuses on gestational days (GD) 16-21 and in neonates and juvenile 
      individuals on postnatal days (PD) 1-28. A double immunostaining method was 
      applied to study the co-localization of the neuronal growth marker 
      growth-associated protein 43 (GAP-43) and the pan-neuronal marker protein gene 
      product 9.5 (PGP 9.5) as well as that of two sensory fibre-associated 
      neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP). The 
      earliest, not yet chemically coded, nerve fibres were observed on GD17 in the 
      perichondrium of the proximal epiphysis. Further development of the innervation 
      was characterized by the successive appearance of nerve fibres in the 
      perichondrium/periosteum of the shaft (GD19), the bone marrow cavity and 
      intercondylar eminence (GD21), the metaphyses (PD1), the cartilage canals 
      penetrating into the epiphyses (PD7), and finally in the secondary ossification 
      centres (PD10) and epiphyseal bone marrow (PD14). Maturation of the fibres, 
      manifested by their immunoreactivity for CGRP and SP, was visible on GD21 in the 
      epiphyseal perichondrium, the periosteum of the shaft and the bone marrow, on PD1 
      in the intercondylar eminence and the metaphyses, on PD7 in the cartilage canals, 
      on PD10 in the secondary ossification centres and on PD14 in the epiphyseal bone 
      marrow. The temporal and topographic pattern of nerve fibre appearance 
      corresponds with the development of regions characterized by active 
      mineralization and bone remodelling, suggesting a possible involvement of the 
      sensory innervation in these processes.
FAU - Gajda, Mariusz
AU  - Gajda M
AD  - Department of Histology, Jagiellonian University, Medical College, Krakow, 
      Poland. mmgajda@cyf-kr.edu.pl
FAU - Litwin, Jan A
AU  - Litwin JA
FAU - Cichocki, Tadeusz
AU  - Cichocki T
FAU - Timmermans, Jean-Pierre
AU  - Timmermans JP
FAU - Adriaensen, Dirk
AU  - Adriaensen D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anat
JT  - Journal of anatomy
JID - 0137162
RN  - 0 (Biomarkers)
RN  - 0 (GAP-43 Protein)
RN  - 33507-63-0 (Substance P)
RN  - EC 3.4.19.12 (UCHL1 protein, rat)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Biomarkers/analysis
MH  - Calcitonin Gene-Related Peptide/analysis
MH  - Embryonic Development/*physiology
MH  - Female
MH  - GAP-43 Protein/analysis
MH  - Gestational Age
MH  - Immunohistochemistry/methods
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Neurons, Afferent/*chemistry
MH  - Osteogenesis
MH  - Rats
MH  - Rats, Wistar
MH  - Substance P/analysis
MH  - Tibia/*embryology/*innervation
MH  - Ubiquitin Thiolesterase/analysis
PMC - PMC1571520
EDAT- 2005/07/30 09:00
MHDA- 2005/11/15 09:00
PMCR- 2007/08/01
CRDT- 2005/07/30 09:00
PHST- 2005/07/30 09:00 [pubmed]
PHST- 2005/11/15 09:00 [medline]
PHST- 2005/07/30 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - JOA434 [pii]
AID - 10.1111/j.1469-7580.2005.00434.x [doi]
PST - ppublish
SO  - J Anat. 2005 Aug;207(2):135-44. doi: 10.1111/j.1469-7580.2005.00434.x.