PMID- 16054141
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20151119
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 579
IP  - 20
DP  - 2005 Aug 15
TI  - Gymnemic acids inhibit rabbit glyceraldehyde-3-phosphate dehydrogenase and induce 
      a smearing of its electrophoretic band and dephosphorylation.
PG  - 4333-6
AB  - Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface 
      plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of 
      the GAPDH band in SDS-PAGE. The GA-induced smearing was diminished by prior 
      incubation of GA with gamma-cyclodextrin or by GA treatment with NAD. GA 
      treatment did not affect the electrophoretic mobility of glucose-6-phosphate 
      isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by 
      an antibody to phosphoserine, but did not affect the phosphoserine bands of 
      glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA 
      specifically induced dephosphorylation of GAPDH.
FAU - Izutani, Yusuke
AU  - Izutani Y
AD  - Graduate School of Agriculture, Kyoto Prefectural University, Kyoto 606-8522, 
      Japan.
FAU - Murai, Takuya
AU  - Murai T
FAU - Imoto, Toshiaki
AU  - Imoto T
FAU - Ohnishi, Masatake
AU  - Ohnishi M
FAU - Oda, Masayuki
AU  - Oda M
FAU - Ishijima, Sumio
AU  - Ishijima S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 327O38FRK1 (gymnemic acid)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
SB  - IM
MH  - Animals
MH  - Electrophoresis
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/*antagonists & inhibitors
MH  - Phosphorylation/drug effects
MH  - Rabbits
MH  - Saponins/*pharmacology
MH  - Triterpenes/*pharmacology
EDAT- 2005/08/02 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/08/02 09:00
PHST- 2005/05/25 00:00 [received]
PHST- 2005/06/21 00:00 [revised]
PHST- 2005/06/26 00:00 [accepted]
PHST- 2005/08/02 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/08/02 09:00 [entrez]
AID - S0014-5793(05)00823-9 [pii]
AID - 10.1016/j.febslet.2005.06.070 [doi]
PST - ppublish
SO  - FEBS Lett. 2005 Aug 15;579(20):4333-6. doi: 10.1016/j.febslet.2005.06.070.