PMID- 16054313
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20071115
IS  - 0378-8741 (Print)
IS  - 0378-8741 (Linking)
VI  - 102
IP  - 3
DP  - 2005 Dec 1
TI  - Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and 
      MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells.
PG  - 418-23
AB  - In this study, we investigated the effects of 95% ethanol (FS-t1), 50% ethanol 
      (FS-t2) and water (FS-w) extracts of Forsythia suspense Vahl (Oleaceae) on the 
      production of regulated on activation, normal T cell expressed and secreted 
      (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza A virus 
      (H1N1)-infected human bronchial epithelial cell line A549. Virus infection evoked 
      a markedly enhanced production of RANTES from basal 16 +/- 4 to 1307 +/- 294 
      pg/ml after 72 h inoculation. At the non-cytotoxic doses (20, 100 and 200 
      microg/ml), FS-t1, FS-t2 and FS-w exhibited a consistent inhibitory effect on 
      virus-stimulated RANTES secretion in a dose-dependent manner wilh IC(50) of 42 
      +/- 6, 117 +/- 15 and 232 +/- 28 microg/ml, respectively. H1N1 also stimulated 
      MCP-1 production in A549 cells, however to a less degree, from basal 133 +/- 21 
      to 391 +/- 98 pg/ml after 72 h viral inoculation. The effects of three extracts 
      on MCP-1 secretion were more complex. FS-t1 displayed both positive and negative 
      effect on virus-stimulated MCP-1 production dependent on the concentrations used. 
      On the other hand, FS-t2 increased virus-induced MCP-1 secretion by 1.4-3.3 times 
      while the third fraction FO-w increased by 2.6-3.7 times. These results suggested 
      that Forsythia suspense consisted of both negative and positive regulatory 
      components on RANTES and MCP-1 secretion in H1N1-infected A549 cells, 
      respectively.
FAU - Ko, Han-Chieh
AU  - Ko HC
AD  - Division of Basic Chinese Medical Research, National Research Institute of 
      Chinese Medicine, Li-Nung St., Shipai, Taipei, Taiwan, ROC.
FAU - Wei, Bai-Luh
AU  - Wei BL
FAU - Chiou, Wen-Fei
AU  - Chiou WF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050727
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Bronchi/*metabolism/virology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemokine CCL5/*biosynthesis
MH  - Dogs
MH  - Epithelial Cells/metabolism/virology
MH  - *Forsythia
MH  - Humans
MH  - Influenza A Virus, H1N1 Subtype/*pathogenicity
MH  - Plant Extracts/*pharmacology
EDAT- 2005/08/02 09:00
MHDA- 2006/03/17 09:00
CRDT- 2005/08/02 09:00
PHST- 2005/05/05 00:00 [received]
PHST- 2005/06/17 00:00 [revised]
PHST- 2005/06/23 00:00 [accepted]
PHST- 2005/08/02 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2005/08/02 09:00 [entrez]
AID - S0378-8741(05)00421-6 [pii]
AID - 10.1016/j.jep.2005.06.029 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2005 Dec 1;102(3):418-23. doi: 10.1016/j.jep.2005.06.029. Epub 
      2005 Jul 27.