PMID- 16054711
OWN - NLM
STAT- MEDLINE
DCOM- 20060313
LR  - 20220409
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1745
IP  - 3
DP  - 2005 Sep 30
TI  - Anisomycin induces COX-2 mRNA expression through p38(MAPK) and CREB independent 
      of small GTPases in intestinal epithelial cells.
PG  - 393-400
AB  - Cyclooxygenase (COX)-2 expression in intestinal epithelial cells is associated 
      with colorectal carcinogenesis. COX-2 expression is induced by numerous growth 
      factors and gastrointestinal hormones through multiple protein kinase cascades. 
      Here, the role of mitogen activated protein kinases (MAPKs) and small GTPases in 
      COX-2 expression was investigated. Anisomycin and sorbitol induced COX-2 
      expression in non-transformed, intestinal epithelial IEC-18 cells. Both 
      anisomycin and sorbitol activated p38(MAPK) followed by phosphorylation of CREB. 
      SB202190 and PD169316 but neither PD98059 nor U0126 blocked COX-2 expression and 
      CREB phosphorylation by anisomycin or sorbitol. Clostridium difficile toxin B 
      inhibition of small GTPases did not affect anisomycin-induced COX-2 mRNA 
      expression or phosphorylation of p38MAPK and CREB but did inhibit 
      sorbitol-dependent COX-2 expression and phosphorylation of p38MAPK and CREB. 
      Angiotensin (Ang) II-dependent induction of COX-2 mRNA and induced 
      phosphorylation of p38MAPK and CREB were inhibited by toxin B. Reduction of CREB 
      protein in cells transfected with CREB siRNAs inhibited anisomycin-induced COX-2 
      expression. These results indicate that activation of p38MAPK signaling is 
      sufficient for COX-2 expression in IEC-18 cells. Ang II and sorbitol require 
      small GTPase activity for COX-2 expression via p38MAPK while anisomycin-induced 
      COX-2 expression by p38MAPK does not require small GTPases. This places small 
      GTPase activity down-stream of the AT1 receptor and hyperosmotic stress and 
      up-stream of p38MAPK and CREB.
FAU - Shafer, Lindsay M
AU  - Shafer LM
AD  - Department of Medicine, David Geffen School of Medicine, CURE: Digestive Diseases 
      Research Center, Jonnson Comprehensive Cancer Center and the Molecular Biology 
      Institute, University of California, Los Angeles, CA 90095-1786, USA.
FAU - Slice, Lee W
AU  - Slice LW
LA  - eng
GR  - DK061485/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Butadienes)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA Primers)
RN  - 0 (Flavonoids)
RN  - 0 (Imidazoles)
RN  - 0 (Nitriles)
RN  - 0 (Pyridines)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (U 0126)
RN  - 0 (toxB protein, Clostridium difficile)
RN  - 506T60A25R (Sorbitol)
RN  - 6C74YM2NGI (Anisomycin)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (Ptgs2 protein, rat)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - GX3Y2V80CV (2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole)
RN  - PVX798P8GI (4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Anisomycin/*pharmacology
MH  - Bacterial Proteins/toxicity
MH  - Bacterial Toxins/toxicity
MH  - Blotting, Western
MH  - Butadienes/pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/genetics/*metabolism
MH  - Cyclooxygenase 2/*metabolism
MH  - DNA Primers
MH  - Flavonoids/pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Imidazoles/pharmacology
MH  - Intestinal Mucosa/*metabolism
MH  - Monomeric GTP-Binding Proteins/antagonists & inhibitors
MH  - Nitriles/pharmacology
MH  - Phosphorylation
MH  - Pyridines/pharmacology
MH  - RNA Interference
MH  - RNA, Messenger/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Signal Transduction/*drug effects
MH  - Sorbitol/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2005/08/02 09:00
MHDA- 2006/03/15 09:00
CRDT- 2005/08/02 09:00
PHST- 2005/04/07 00:00 [received]
PHST- 2005/07/01 00:00 [revised]
PHST- 2005/07/06 00:00 [accepted]
PHST- 2005/08/02 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2005/08/02 09:00 [entrez]
AID - S0167-4889(05)00151-5 [pii]
AID - 10.1016/j.bbamcr.2005.07.002 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2005 Sep 30;1745(3):393-400. doi: 
      10.1016/j.bbamcr.2005.07.002.