PMID- 16055005
OWN - NLM
STAT- MEDLINE
DCOM- 20051123
LR  - 20060425
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 39
IP  - 2
DP  - 2005 Aug
TI  - The involvement of immunoglobulin E isotype switch in scleroderma skin tissue.
PG  - 97-104
AB  - BACKGROUND: The involvement of mast cell, which is activated by immunoglobulin E 
      (IgE), has been reported in the formation of systemic sclerosis (SSc) 
      abnormality. IgE is generated with isotype switch. During isotype switch, switch 
      circles resulting from direct mu to epsilon, or from sequential mu to gamma via 
      epsilon switching will be created. OBJECTIVE: We studied whether switching occurs 
      in SSc. METHODS: We used nested polymerase chain reaction to analyze the S 
      fragments from switch circles. Fifty-two patients with SSc, and 62 healthy women 
      were studied. RESULTS: Neither of 62 normal skin tissues showed direct switch, 
      nor sequential switch. Neither of seven normal whole blood cells showed direct 
      switch, nor sequential switch. In 52SSc skin tissues, three (5.8%) showed direct 
      switch, and two (3.8%) showed sequential switch. As a result, five (9.6%) of SSc 
      skin tissue showed immunogobulin E class switch. These results were confirmed by 
      DNA sequencing. CONCLUSION: These results demonstrated that isotype switch to the 
      epsilon locus achieved by direct and/or sequential switch are involved in SSc 
      skin.
FAU - Ohtsuka, Tsutomu
AU  - Ohtsuka T
AD  - Department of Dermatology, Koshigaya Hospital, Dokkyo University School of 
      Medicine, Koshigaya, Saitama, Japan. tohtsuka@dokkyomed.ac.jp
FAU - Yamazaki, Soji
AU  - Yamazaki S
LA  - eng
PT  - Journal Article
DEP - 20050415
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Immunoglobulin epsilon-Chains)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Female
MH  - Humans
MH  - Immunoglobulin Class Switching
MH  - Immunoglobulin E/*chemistry/genetics
MH  - Immunoglobulin Isotypes/*chemistry
MH  - Immunoglobulin epsilon-Chains/chemistry
MH  - Immunoglobulin mu-Chains/chemistry
MH  - Middle Aged
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Recombination, Genetic
MH  - Scleroderma, Systemic/*immunology/metabolism/pathology
MH  - Sequence Analysis, DNA
MH  - Skin/immunology/pathology
EDAT- 2005/08/02 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/02 09:00
PHST- 2004/11/11 00:00 [received]
PHST- 2005/02/10 00:00 [revised]
PHST- 2005/02/15 00:00 [accepted]
PHST- 2005/08/02 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/02 09:00 [entrez]
AID - S0923-1811(05)00075-7 [pii]
AID - 10.1016/j.jdermsci.2005.02.001 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2005 Aug;39(2):97-104. doi: 10.1016/j.jdermsci.2005.02.001. Epub 
      2005 Apr 15.