PMID- 16061391
OWN - NLM
STAT- MEDLINE
DCOM- 20051018
LR  - 20051117
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 31
IP  - 5
DP  - 2005 Sep 7
TI  - Comparative activity of Sant7 and anti-IL-6, IL-6R monoclonal antibodies in a 
      murine model of B-cell lymphoma.
PG  - 368-74
AB  - Interleukin-6 (IL-6) plays a central role in the pathogenesis of several 
      autoimmune and inflammatory diseases as well as B-cell lymphoproliferative 
      disorders. This work describes the effects of the recombinant or 
      adenovirally-delivered IL-6 superantagonist Sant7, anti-IL-6 and IL-6 receptor 
      monoclonal antibodies in a severe murine model of human B-cell lymphoma induced 
      in SCID mice by transplantation of an LCL-41 cell line variant (isotype-switched 
      IgM>IgG). Survival of 60% of the animals treated with anti-gp130 was observed up 
      to day 33, while about 20% of the animals survived with anti-gp80 and Sant7 
      treatment. No survival was observed with the anti-IL-6 monoclonal antibody 
      treatment. No significant change in serum and peritoneal levels of human IL-6 
      (hIL-6) and soluble human IL-6 receptor (shIL-6R) was observed in the recombinant 
      Sant7-treated group towards the control group. The anti-gp80 monoclonal antibody 
      induced significant increase of both hIL-6R and hIL-6 in serum and peritoneum. 
      The anti-gp130 monoclonal antibody treatment determined a reduction of the seric 
      shIL-6R and a significant increase of the seric hIL-6. Anti-IL-6 monoclonal 
      antibody administration resulted in a reduction of serum and in an increase of 
      peritoneal hIL-6. Treatment with adenoviral Sant7 was associated with a reduction 
      of circulating shIL-6R, hIgG and mSAP. However, only marginal anti-tumor efficacy 
      of the adenoviral Sant7 was observed. Overall, the present data suggest a 
      potential for anti-hIL-6 therapy in B-cell lymphomas. Less severe animal models 
      might be useful to better evaluate Sant7 efficacy alone or in combination with 
      other anti-IL-6 therapeutics.
FAU - Campo, Silvia
AU  - Campo S
AD  - Department of Immunology, Sigma-Tau SpA R&D, Via Pontina Km 30400, Pomezia 00040, 
      Rome, Italy. silvia.campo@sigma-tau.it
FAU - Serlupi-Crescenzi, Ottaviano
AU  - Serlupi-Crescenzi O
FAU - Arseni, Brunilde
AU  - Arseni B
FAU - Rossi, Stefania
AU  - Rossi S
FAU - Saggio, Isabella
AU  - Saggio I
FAU - Salone, B
AU  - Salone B
FAU - Cherubini, G
AU  - Cherubini G
FAU - Carminati, Paolo
AU  - Carminati P
FAU - De Santis, Rita
AU  - De Santis R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Complementary)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Il6st protein, mouse)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SANT-7)
RN  - 133483-10-0 (Cytokine Receptor gp130)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Antibodies, Monoclonal/*chemistry
MH  - Antigens, CD/biosynthesis
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cytokine Receptor gp130
MH  - DNA, Complementary/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Immunoglobulin G/chemistry
MH  - Inflammation
MH  - Interleukin-6/*analogs & derivatives/biosynthesis/blood/*immunology/metabolism
MH  - Lymphoma, B-Cell/immunology/*metabolism
MH  - Membrane Glycoproteins/biosynthesis
MH  - Mice
MH  - Mice, SCID
MH  - Neoplasm Transplantation
MH  - Receptors, Interleukin-6/biosynthesis/*immunology
MH  - Recombinant Proteins/chemistry
MH  - Time Factors
EDAT- 2005/08/03 09:00
MHDA- 2005/10/19 09:00
CRDT- 2005/08/03 09:00
PHST- 2004/12/03 00:00 [received]
PHST- 2005/06/01 00:00 [revised]
PHST- 2005/06/13 00:00 [accepted]
PHST- 2005/08/03 09:00 [pubmed]
PHST- 2005/10/19 09:00 [medline]
PHST- 2005/08/03 09:00 [entrez]
AID - S1043-4666(05)00196-1 [pii]
AID - 10.1016/j.cyto.2005.06.006 [doi]
PST - ppublish
SO  - Cytokine. 2005 Sep 7;31(5):368-74. doi: 10.1016/j.cyto.2005.06.006.