PMID- 16075199
OWN - NLM
STAT- MEDLINE
DCOM- 20060209
LR  - 20161124
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 184
IP  - 1
DP  - 2005 Oct
TI  - Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in 
      Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis.
PG  - 41-8
AB  - Formaldehyde activating enzyme (Fae) was first discovered in methylotrophic 
      bacteria, where it is involved in the oxidation of methanol to CO2 and in 
      formaldehyde detoxification. The 18 kDa protein catalyzes the condensation of 
      formaldehyde with tetrahydromethanopterin (H4MPT) to methylene-H4MPT. We describe 
      here that Fae is also present and functional in the methanogenic archaeon 
      Methanosarcina barkeri. The faeA homologue in the genome of M. barkeri was 
      heterologously expressed in Escherichia coli and the overproduced purified 
      protein shown to actively catalyze the condensation reaction: apparent Vmax = 13 
      U/mg protein (1 U = micromol/min); apparent Km for H4MPT = 30 microM; apparent Km 
      for formaldehyde = 0.1 mM. By Western blot analysis the concentration of Fae in 
      cell extracts of M. barkeri was determined to be in the order of 0.1% of the 
      soluble cell proteins. Besides the faeA gene the genome of M. barkeri harbors a 
      second gene, faeB-hpsB, which is shown to code for a 42 kDa protein with both Fae 
      activity (3.6 U/mg) and hexulose-6-phosphate synthase (Hps) activity (4.4 U/mg). 
      The results support the recent proposal that in methanogenic archaea Fae and Hps 
      could have a function in ribose phosphate synthesis.
FAU - Goenrich, Meike
AU  - Goenrich M
AD  - Max-Planck-Institut fur terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, 
      35043, Marburg, Germany.
FAU - Thauer, Rudolf K
AU  - Thauer RK
FAU - Yurimoto, Hiroya
AU  - Yurimoto H
FAU - Kato, Nobuo
AU  - Kato N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051103
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Ribosemonophosphates)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 4B2428FLTO (ribose-5-phosphate)
RN  - EC 4.1.2.- (Aldehyde-Lyases)
RN  - EC 4.1.2.- (hexose phosphate synthetase)
RN  - EC 6.3.- (Carbon-Nitrogen Ligases)
SB  - IM
MH  - Aldehyde-Lyases/chemistry/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Carbon-Nitrogen Ligases/chemistry/genetics/*metabolism
MH  - Formaldehyde/chemistry/metabolism
MH  - Methanosarcina barkeri/enzymology/genetics/growth & development/*metabolism
MH  - Molecular Sequence Data
MH  - Ribosemonophosphates/*biosynthesis
EDAT- 2005/08/03 09:00
MHDA- 2006/02/10 09:00
CRDT- 2005/08/03 09:00
PHST- 2005/04/06 00:00 [received]
PHST- 2005/05/12 00:00 [accepted]
PHST- 2005/04/29 00:00 [revised]
PHST- 2005/08/03 09:00 [pubmed]
PHST- 2006/02/10 09:00 [medline]
PHST- 2005/08/03 09:00 [entrez]
AID - 10.1007/s00203-005-0008-1 [doi]
PST - ppublish
SO  - Arch Microbiol. 2005 Oct;184(1):41-8. doi: 10.1007/s00203-005-0008-1. Epub 2005 
      Nov 3.