PMID- 16075371
OWN - NLM
STAT- MEDLINE
DCOM- 20051209
LR  - 20181113
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 16
IP  - 6
DP  - 2005 Jun
TI  - Construction of a cytogenetically anchored microsatellite map in rabbit.
PG  - 442-59
AB  - Rabbit (Oryctolagus cuniculus) represents a valuable source of biomedical models 
      and corresponds to a small but active economic sector in Europe for meat and fur. 
      The rabbit genome has not been thoroughly studied until recently, and 
      high-resolution maps necessary for identification of genes and quantitative trait 
      loci (QTL) are not yet available. Our aim was to isolate over 300 new and 
      regularly distributed (TG)n or (TC)n rabbit microsatellites. To achieve this 
      purpose, 164 microsatellite sequences were isolated from gene-containing 
      bacterial artificial chromosome (BAC) clones previously localized by fluorescence 
      in situ hybridization (FISH) on all the rabbit chromosomes. In addition, 141 
      microsatellite sequences were subcloned from a plasmid genomic library, and for 
      41 of these sequences, BAC clones were identified and FISH-mapped. TC repeats 
      were present in 62% of the microsatellites derived from gene-containing BAC 
      clones and in 22% of those from the plasmid genomic library, with an average of 
      42.9% irrespective of the microsatellite origin. These results suggest a higher 
      proportion of (TC)n repeats and a nonhomogeneous distribution of (TG)n and (TC)n 
      repeats in the rabbit genome compared to those in man. Among the 305 isolated 
      microsatellites, 177 were assigned to 139 different cytogenetic positions on all 
      the chromosomes except rabbit Chromosome 21. Sequence similarity searches 
      provided hit locations on the Human Build 35a and hypothetical assignments on 
      rabbit chromosomes for ten additional microsatellites. Taken together, these 
      results report a reservoir of 305 new rabbit microsatellites of which 60% have a 
      cytogenetic position. This is the first step toward the construction of an 
      integrated cytogenetic and genetic map based on microsatellites homogeneously 
      anchored to the rabbit genome.
FAU - Chantry-Darmon, Celine
AU  - Chantry-Darmon C
AD  - Laboratoire de Radiobiologie et d'Etude du Genome, Unite Mixte de Recherche, 
      Institut National de la Recherche Agronomique, Commissariat a l'Energie Atomique 
      13.314, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
FAU - Urien, Celine
AU  - Urien C
FAU - Hayes, Helene
AU  - Hayes H
FAU - Bertaud, Maud
AU  - Bertaud M
FAU - Chadi-Taourit, Sead
AU  - Chadi-Taourit S
FAU - Chardon, Patrick
AU  - Chardon P
FAU - Vaiman, Daniel
AU  - Vaiman D
FAU - Rogel-Gaillard, Claire
AU  - Rogel-Gaillard C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - Cytogenetics/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microsatellite Repeats/*genetics
MH  - Rabbits/*genetics
MH  - Sequence Homology
EDAT- 2005/08/03 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/03 09:00
PHST- 2005/01/18 00:00 [received]
PHST- 2005/03/18 00:00 [accepted]
PHST- 2005/08/03 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/03 09:00 [entrez]
AID - 10.1007/s00335-005-2471-z [doi]
PST - ppublish
SO  - Mamm Genome. 2005 Jun;16(6):442-59. doi: 10.1007/s00335-005-2471-z.