PMID- 16076300
OWN - NLM
STAT- MEDLINE
DCOM- 20051103
LR  - 20191210
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 60
IP  - 9
DP  - 2005 Sep
TI  - Validation of basophil histamine release against the autologous serum skin test 
      and outcome of serum-induced basophil histamine release studies in a large 
      population of chronic urticaria patients.
PG  - 1152-6
AB  - BACKGROUND: Endogenous histamine-releasing factors (HRFs) are involved in 30-60% 
      of patients with chronic urticaria (CU). Evidence for their existence comes from 
      in vivo studies of autoreactivity with the autologous serum skin test (ASST), in 
      vitro immunoassays demonstrating autoantibodies against the immunoglobulin E 
      (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine 
      release (HR) from basophils and mast cells. We have examined the correlation 
      between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria 
      test) in a group of well-characterized CU patients and subsequently determined 
      the frequency of HR-Urticaria-positive sera from a larger population of CU 
      patients. SUBJECTS: Group 1 consisted of 28 patients with CU (16 were 
      ASST-positive) 20 patients with atopic dermatitis, 24 patients with allergy to 
      birch and nine healthy controls. Group 2 consisted of 873 unselected CU patients. 
      METHODS: White blood cells containing 1-2% basophils from a healthy nonatopic 
      donor were incubated with patients sera in the presence of interleukin (IL)-3. 
      Histamine was measured by the glass fibre method. RESULTS: Using the ASST as the 
      true outcome, the HR-Urticaria test showed a sensitivity and specificity of 75% 
      in group 1 using a cut-off value for HR of >16.5%. None of the controls was 
      positive in the HR-Urticaria test. In group 2, we found no difference in the 
      frequency of positives between male (34.6%, n = 254) and female adults (35.1%, n 
      = 576) but twice as many females as males were tested. CONCLUSIONS: Our studies 
      have shown that the HR-Urticaria test has a good sensitivity and specificity for 
      endogenous HRFs demonstrated by the ASST in patients with CU and that about 
      one-third of unselected patients with CU have a positive result.
FAU - Platzer, M H
AU  - Platzer MH
AD  - National University Hospital, Allergy Clinic, Laboratory of Medical Allergology, 
      Copenhagen, Denmark.
FAU - Grattan, C E H
AU  - Grattan CE
FAU - Poulsen, L K
AU  - Poulsen LK
FAU - Skov, P S
AU  - Skov PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Autoantibodies)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Autoantibodies/immunology
MH  - Basophils/immunology/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Female
MH  - Histamine/*biosynthesis
MH  - Humans
MH  - Immunoassay
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - *Skin Tests
MH  - Urticaria/*immunology
EDAT- 2005/08/04 09:00
MHDA- 2005/11/04 09:00
CRDT- 2005/08/04 09:00
PHST- 2005/08/04 09:00 [pubmed]
PHST- 2005/11/04 09:00 [medline]
PHST- 2005/08/04 09:00 [entrez]
AID - ALL841 [pii]
AID - 10.1111/j.1398-9995.2005.00841.x [doi]
PST - ppublish
SO  - Allergy. 2005 Sep;60(9):1152-6. doi: 10.1111/j.1398-9995.2005.00841.x.