PMID- 16077905
OWN - NLM
STAT- MEDLINE
DCOM- 20051205
LR  - 20190513
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 37
IP  - 8
DP  - 2005 Aug
TI  - Sequence-specific assignment of 1H-NMR resonance and determination of the 
      secondary structure of Jingzhaotoxin-I.
PG  - 567-72
AB  - Jingzhaotoxin-I (JZTX-I) purified from the venom of the spider Chilobrachys 
      jingzhao is a novel neurotoxin preferentially inhibiting cardiac sodium channel 
      inactivation by binding to receptor site 3. The structure of this toxin in 
      aqueous solution was investigated using 2-D 1H-NMR techniques. The complete 
      sequence-specific assignments of proton resonance in the 1H-NMR spectra of JZTX-I 
      were obtained by analyzing a series of 2-D spectra, including DQF-COSY, TOCSY and 
      NOESY spectra, in H2O and D2O. All the backbone protons except for Gln4 and more 
      than 95% of the side-chain protons were identified by d alphaN, d alphadelta, d 
      betaN and d NN connectivities in the NOESY spectrum. These studies provide a 
      basis for the further determination of the solution conformation of JZTX-I. 
      Furthermore, the secondary structure of JZTX-I was identified from NMR data. It 
      consists mainly of a short triple-stranded antiparallel beta-sheet with 
      Trp7-Cys9, Phe20-Lys23 and Leu28-Trp31. The characteristics of the secondary 
      structure of JZTX-I are similar to those of huwentoxin-I (HWTX-I) and 
      hainantoxin-IV (HNTX-IV), whose structures in solution have previously been 
      reported.
FAU - Zeng, Xiong-Zhi
AU  - Zeng XZ
AD  - College of Life Sciences, Hunan Normal University, Changsha 410081, China.
FAU - Zhu, Qi
AU  - Zhu Q
FAU - Liang, Song-Ping
AU  - Liang SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Peptides)
RN  - 0 (Protons)
RN  - 0 (Spider Venoms)
RN  - 0 (jingzhaotoxin-I, Chilobrachys jingzhao)
SB  - IM
MH  - Amino Acid Sequence
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Molecular Sequence Data
MH  - Peptide Mapping/*methods
MH  - Peptides/*analysis/*chemistry
MH  - Protein Structure, Secondary
MH  - Protons
MH  - Sequence Analysis, Protein/*methods
MH  - Spider Venoms/*analysis/*chemistry
EDAT- 2005/08/04 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/04 09:00
PHST- 2005/08/04 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/04 09:00 [entrez]
AID - 10.1111/j.1745-7270.2005.00078.x [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2005 Aug;37(8):567-72. doi: 
      10.1111/j.1745-7270.2005.00078.x.