PMID- 16077989
OWN - NLM
STAT- MEDLINE
DCOM- 20050915
LR  - 20181201
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 14
IP  - 3
DP  - 2005 Sep
TI  - Generation of diverse mutated tumor antigen-specific cytotoxic T lymphocytes in a 
      lung cancer patient with long survival.
PG  - 763-9
AB  - We have identified an antigen recognized on a large cell carcinoma of the lung by 
      tumor-specific cytotoxic T lymphocytes (CTL). The antigenic peptide is encoded by 
      a mutated alpha-actinin-4 gene and presented by human leukocyte antigen (HLA)-A2. 
      Using HLA-A2-peptide tetramers, we have derived from patient peripheral blood 
      lymphocytes (PBL) and autologous tumor infiltrating lymphocytes (TIL) several 
      mutated alpha-actinin-4-specific T cell clones. These clones displayed similar 
      tetramer staining but distinct T cell receptor (TCR) usage and antitumor 
      reactivity. Indeed, TIL clones lysed more efficiently the autologous tumor cells 
      and released higher cytokine levels than PBL clones. Importantly, treatment of 
      cancer cells with interferon-gamma enhanced their susceptibility to PBL 
      clone-mediated lysis correlated with increase in HLA-class I expression. The 
      present findings provide evidence that an immune T cell response took place in a 
      lung cancer patient with favorable clinical evolution and suggest that CTL, 
      recognizing a truly tumor-specific antigen, may contribute to controlling the 
      tumor.
FAU - El Hage, Faten
AU  - El Hage F
AD  - Laboratoire Cytokines et Immunologie des Tumeurs Humaines, U487 INSERM, Institut 
      Federatif de Recherche-54, F-94 805 Villejuif, France.
FAU - Vergnon, Isabelle
AU  - Vergnon I
FAU - Grunenwald, Dominique
AU  - Grunenwald D
FAU - Soria, Jean Charles
AU  - Soria JC
FAU - Chouaib, Salem
AU  - Chouaib S
FAU - Mami-Chouaib, Fathia
AU  - Mami-Chouaib F
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Cytokines)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Microfilament Proteins)
RN  - 11003-00-2 (Actinin)
SB  - IM
MH  - Actinin/*genetics/immunology
MH  - Aged
MH  - Carcinoma, Large Cell/genetics/immunology/*pathology
MH  - Cytokines/metabolism
MH  - Cytotoxicity Tests, Immunologic
MH  - Cytotoxicity, Immunologic/immunology
MH  - Flow Cytometry
MH  - Follow-Up Studies
MH  - HLA-A2 Antigen/immunology
MH  - Humans
MH  - Lung Neoplasms/genetics/immunology/*pathology
MH  - Male
MH  - Microfilament Proteins/*genetics/immunology
MH  - Mutation
MH  - T-Lymphocytes, Cytotoxic/*immunology/metabolism/pathology
EDAT- 2005/08/04 09:00
MHDA- 2005/09/16 09:00
CRDT- 2005/08/04 09:00
PHST- 2005/08/04 09:00 [pubmed]
PHST- 2005/09/16 09:00 [medline]
PHST- 2005/08/04 09:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2005 Sep;14(3):763-9.