PMID- 16080198
OWN - NLM
STAT- MEDLINE
DCOM- 20051206
LR  - 20071115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 44
IP  - 4
DP  - 2005 Dec
TI  - Genomic profiling of myeloid sarcoma by array comparative genomic hybridization.
PG  - 373-83
AB  - Myeloid sarcoma (MS) is a tumor mass of myeloblasts or immature myeloid cells 
      occurring in an extramedullary site. In this study, seven cases of MS [stomach 
      (1), testis (1), skin (2), and lymph node (3)] and 3 synchronous and 1 follow-up 
      bone marrow (BM) samples were studied for genomic abnormalities using array 
      comparative genomic hybridization (array-CGH). Array-CGH construction used 
      approximately 5,400 bacterial artificial chromosome clones from the RPCI-11 
      library, spanning the human genome. Data were analyzed using the DNAcopy software 
      and custom heuristics. All MS cases had genomic abnormalities detected by 
      array-CGH. Unbalanced genomic abnormalities in five MS cases were confirmed by 
      conventional cytogenetics (CC) and/or fluorescence in situ hybridization (FISH); 
      these abnormalities included loss of 4q32.1-q35.2, 6q16.1-q21, and 12p12.2-p13.2 
      and gain of 8q21.2-q24.3, 8, 11q21-q25, 13q21.32-q34, 19, and 21. Array-CGH was 
      also invaluable in identifying possible deletions, partner translocations, and 
      breakpoints that were questionable by CC. The remaining two MS cases had genomic 
      aberrations detected by array-CGH, but were not studied further by CC/FISH. 
      Chromosome 8 was most commonly abnormal (3/7 cases). Identical genomic 
      abnormalities were demonstrated in MS and in synchronous BM in two cases. These 
      results demonstrate that array-CGH is a powerful tool to screen MS tissue for 
      unbalanced genomic abnormalities, allowing identification of chromosome 
      abnormalities when concurrent BM is nonanalyzable or nonleukemic.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Deeb, George
AU  - Deeb G
AD  - Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute, 
      Buffalo, NY 14263, USA. george.deeb@roswellpark.org
FAU - Baer, Maria R
AU  - Baer MR
FAU - Gaile, Daniel P
AU  - Gaile DP
FAU - Sait, Sheila N Jani
AU  - Sait SN
FAU - Barcos, Maurice
AU  - Barcos M
FAU - Wetzler, Meir
AU  - Wetzler M
FAU - Conroy, Jeffrey M
AU  - Conroy JM
FAU - Nowak, Norma J
AU  - Nowak NJ
FAU - Cowell, John K
AU  - Cowell JK
FAU - Cheney, Richard T
AU  - Cheney RT
LA  - eng
GR  - CAA16056-27/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosomes, Artificial, Bacterial
MH  - Cytogenetic Analysis
MH  - DNA/*analysis
MH  - Female
MH  - Gene Dosage
MH  - *Gene Expression Profiling
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Microarray Analysis
MH  - Middle Aged
MH  - *Nucleic Acid Hybridization
MH  - Sarcoma, Myeloid/*genetics/pathology
MH  - Sensitivity and Specificity
EDAT- 2005/08/05 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/05 09:00
PHST- 2005/08/05 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/05 09:00 [entrez]
AID - 10.1002/gcc.20239 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2005 Dec;44(4):373-83. doi: 10.1002/gcc.20239.