PMID- 16080847
OWN - NLM
STAT- MEDLINE
DCOM- 20060609
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 44
IP  - 7
DP  - 2005 Jul
TI  - [Peritubular capillary injury in Chinese herb guan-mu-tong-induced acute tubular 
      necrosis].
PG  - 525-9
AB  - OBJECTIVE: To explore the role and mechanisms of peritubular capillary (PTC) 
      injury in the progression of Chinese Herb guan-mu-tong (GMT, aristolochiae 
      manshuriensis kom) induced acute tubular necrosis (GMT-ATN). METHODS: Renal 
      biopsy tissue from 4 cases of GMT-ATN and 5 cases of antibiotic induced ATN 
      (A-ATN) were included in the study. Tubulointerstitial injury was 
      semi-quantitatively assessed. Immunohistochemical SP method was applied to reveal 
      PTC as well as the expression of vascular endothelial growth factor (VEGF). Ultra 
      microstructure of endothelial cells and basement membrane of PTC was detected by 
      electronic microscopy (EM). 5 cases of minor mesangioproliferative non-IgA 
      glomerulonephritis were selected as a control group. RESULTS: The density of PTC 
      was decreased significantly in GMT-ATN, as compared with the A-ATN and control 
      group (211.08 +/- 56.15 vs 413.54 +/- 66.59, 536.62 +/- 68.38, P < 0.01). Dilated 
      and deformed PTC lumina were noted in GMT-ATN with some endothelial cells and 
      basement membrane partially disrupted. Most endothelial cells were found to be 
      swollen with vacuoles dispersed in the cell plasma. The basement membrane was 
      partially shrunk and thickened. The expression of VEGF in renal tubular 
      epithelial cells (RTEC) was much less in the GMT-ATN than that in A-ATN group 2.1 
      (0-3.86)% vs [42.5 (31.33-60.25)%, P < 0.01], even though it was higher than that 
      in the control group [23.1 (18.2-39.5)%, P < 0.01]; the expression was correlated 
      with PTC density. Close correlation was also found between RTEC regeneration and 
      PTC density, as well as VEGF expression (r = 0.880 and 0.802 respectively, P < 
      0.01). CONCLUSIONS: PTC was markedly injured in GMT-ATN; this could be one of the 
      cause for the continuously progressing tubulointerstitial damage. The low 
      expression of VEGF in RTEC might contribute to the PTC injury process.
FAU - Yang, Li
AU  - Yang L
AD  - Institute of Nephrology and Division of Nephrology, First Hospital, Peking 
      University, Beijing 100034, China.
FAU - Li, Xiao-mei
AU  - Li XM
FAU - Wang, Su-xia
AU  - Wang SX
FAU - Wang, Hai-yan
AU  - Wang HY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - *Aristolochia/chemistry
MH  - Basement Membrane/ultrastructure
MH  - Capillaries/pathology
MH  - Drugs, Chinese Herbal/*adverse effects/isolation & purification
MH  - Endothelial Cells/ultrastructure
MH  - Female
MH  - Humans
MH  - Immunochemistry
MH  - Kidney Tubular Necrosis, Acute/*chemically induced/metabolism/pathology
MH  - Kidney Tubules/*blood supply
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2005/08/06 09:00
MHDA- 2006/06/10 09:00
CRDT- 2005/08/06 09:00
PHST- 2005/08/06 09:00 [pubmed]
PHST- 2006/06/10 09:00 [medline]
PHST- 2005/08/06 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2005 Jul;44(7):525-9.