PMID- 16080959
OWN - NLM
STAT- MEDLINE
DCOM- 20050915
LR  - 20060406
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 161
IP  - 1
DP  - 2005 Aug
TI  - The human ovarian teratocarcinoma cell line PA-1 demonstrates a single 
      translocation: analysis with fluorescence in situ hybridization, spectral 
      karyotyping, and bacterial artificial chromosome microarray.
PG  - 63-9
AB  - Cell lines derived from tumors contain numerous chromosomal aberrations and are 
      the focus of study in tumor evolution. The ovarian teratocarcinoma cell line PA-1 
      demonstrates a single chromosomal aberration: a reciprocal t(15;20)(p11.2;q11.2). 
      A complete molecular genetic analysis was undertaken to characterize this cell 
      line. The PA-1 cell line was studied with fluorescence in situ hybridization 
      (FISH), spectral karyotyping (SKY), bacterial artificial chromosome (BAC) 
      microarray, and Western blotting. Amplification of 20q is frequently implicated 
      in both breast and ovarian cancer; this region contains a number of oncogenes 
      including MDM2, ZNF217, and the ovarian tumor marker WFDC2 (alias HE4). FISH 
      revealed gene amplification of AIB1 (now known as NCOA3) but not STK15 (now known 
      as AURKA). Immunoblot analysis demonstrated 3.6-fold overexpression of the AIB1 
      protein product, but no elevation of the STK15. BAC cancer gene microarray 
      analysis showed gene amplification of > or =1.20 for five oncogenes. The presence 
      of a consistent single change in PA-1, the t(15;20)(p11.2;q11.2), suggests that 
      the aberration is significant with respect to the transformation status of the 
      cell line. This translocation appears to cause overexpression of AIB1 (and 
      perhaps other proteins), which may provide an immortalizing effect on this cell 
      line.
FAU - Sarraf, Shireen
AU  - Sarraf S
AD  - Department of Oncology, Georgetown University, 3800 Reservoir Road NW, 
      Washington, DC 20007, USA.
FAU - Tejada, Raphael
AU  - Tejada R
FAU - Abawi, Massih
AU  - Abawi M
FAU - Oberst, Michael
AU  - Oberst M
FAU - Dennis, Tom
AU  - Dennis T
FAU - Simon, Kelly Claire
AU  - Simon KC
FAU - Blancato, Jan
AU  - Blancato J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Blotting, Western
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Chromosomes, Human, Pair 20/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microarray Analysis
MH  - Oncogenes/physiology
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - Spectral Karyotyping
MH  - Teratocarcinoma/*genetics/pathology
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2005/08/06 09:00
MHDA- 2005/09/16 09:00
CRDT- 2005/08/06 09:00
PHST- 2004/09/30 00:00 [received]
PHST- 2004/12/15 00:00 [revised]
PHST- 2005/01/04 00:00 [accepted]
PHST- 2005/08/06 09:00 [pubmed]
PHST- 2005/09/16 09:00 [medline]
PHST- 2005/08/06 09:00 [entrez]
AID - S0165-4608(05)00017-8 [pii]
AID - 10.1016/j.cancergencyto.2005.01.003 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Aug;161(1):63-9. doi: 
      10.1016/j.cancergencyto.2005.01.003.