PMID- 16080961
OWN - NLM
STAT- MEDLINE
DCOM- 20050915
LR  - 20220316
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 161
IP  - 1
DP  - 2005 Aug
TI  - Chronic lymphocytic leukemia developing in a patient with chronic myeloid 
      leukemia: evidence of distinct lineage-associated genomic events.
PG  - 74-7
AB  - We describe the cytogenetic, fluorescence in situ hybridization (FISH), and 
      molecular findings in a patient who developed a typical chronic lymphocytic 
      leukemia (CLL) 20 months after the diagnosis of a Philadelphia (Ph)-positive 
      chronic myeloid leukemia. Unstimulated bone marrow culture showed a 
      46,XX,t(9;22)(q34;q11) karyotype, and interphase FISH detected the presence of a 
      BCR/ABL fusion signal in 13% of cells. On stimulated bone marrow culture, a 
      normal karyotype and a 13q14 deletion by interphase FISH with D13S319 probe in 
      14% of the cells were found. Molecular studies detected the chimeric BCR/ABL 
      messengers by nested reverse-transcriptase polymerase chain reaction. The 
      B-cellular clone was documented by the presence of a clonal heavy chain 
      immunoglobulin rearrangement. The coexistence of these two hematologic 
      malignancies leads to questions about their cell(s) of origin. We provide 
      evidence that CLL arose in a Ph-negative clone. The implications of these 
      findings are discussed.
FAU - Gargallo, Patricia
AU  - Gargallo P
AD  - Departamento de Genetica, Academia Nacional de Medicina, Pacheco de Melo 3081, 
      Buenos Aires 1425, Argentina.
FAU - Cacchione, Roberto
AU  - Cacchione R
FAU - Chena, Christian
AU  - Chena C
FAU - Dupont, Juan
AU  - Dupont J
FAU - Garay, Guy
AU  - Garay G
FAU - Riveros, Dardo
AU  - Riveros D
FAU - Larripa, Irene
AU  - Larripa I
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Lymphocytes/pathology
MH  - Cell Lineage
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/pathology
MH  - Neoplasms, Second Primary/*genetics/pathology
MH  - Philadelphia Chromosome
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
RF  - 33
EDAT- 2005/08/06 09:00
MHDA- 2005/09/16 09:00
CRDT- 2005/08/06 09:00
PHST- 2004/12/16 00:00 [received]
PHST- 2005/01/11 00:00 [accepted]
PHST- 2005/08/06 09:00 [pubmed]
PHST- 2005/09/16 09:00 [medline]
PHST- 2005/08/06 09:00 [entrez]
AID - S0165-4608(05)00046-4 [pii]
AID - 10.1016/j.cancergencyto.2005.01.008 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Aug;161(1):74-7. doi: 
      10.1016/j.cancergencyto.2005.01.008.