PMID- 16081932
OWN - NLM
STAT- MEDLINE
DCOM- 20050915
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 43
IP  - 8
DP  - 2005 Aug
TI  - Bifidobacterial lipoglycan as a new cause for false-positive platelia Aspergillus 
      enzyme-linked immunosorbent assay reactivity.
PG  - 3925-31
AB  - We previously hypothesized that a lipoglycan of Bifidobacterium bifidum subsp. 
      pennsylvanicum cross-reacts with the Platelia Aspergillus (PA) enzyme-linked 
      immunosorbent assay (ELISA) based on the presence of galactofuranosyl epitopes in 
      the cell wall (M. A. S. H. Mennink-Kersten, R. R. Klont, A. Warris, H. J. M. Op 
      den Camp, and P. E. Verweij, Lancet 363:325-327, 2004). We tested this hypothesis 
      by testing bacterial suspensions of different bifidobacterial species and other 
      gram-positive and -negative bacteria with the PA ELISA, which is used to detect 
      circulating galactomannan for the serodiagnosis of invasive aspergillosis. 
      Furthermore, neonatal fecal samples were enumerated for bifidobacteria by 
      fluorescence in situ hybridization (FISH) and tested for PA ELISA reactivity. All 
      bifidobacteria, except B. infantis and B. adolescentis, showed reactivity 6- to 
      600-fold higher compared to the controls (i.e., Micrococcus luteus and 
      Propionibacterium freudenreichii, which contain a cell wall lipomannan). 
      Eggerthella lenta showed a 25-fold-higher reactivity. ELISA reactivity was 
      clearly shown to be associated with bacterial lipoglycans containing a 
      beta-1,5-galactofuranosyl chain. All neonatal feces showed PA ELISA reactivity 
      and associated numbers of bifidobacteria. Since high concentrations of 
      bifidobacteria are present in the human gut, these bacteria or excreted 
      lipoglycan may cause false serum PA ELISA reactivity in selected patient groups, 
      especially neonates.
FAU - Mennink-Kersten, Monique A S H
AU  - Mennink-Kersten MA
AD  - Department of Medical Microbiology, Radboud University Nijmegen Medical Center, 
      P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. m.mennink@mmb.umcn.nl
FAU - Ruegebrink, Dorien
AU  - Ruegebrink D
FAU - Klont, Rocus R
AU  - Klont RR
FAU - Warris, Adilia
AU  - Warris A
FAU - Gavini, Francoise
AU  - Gavini F
FAU - Op den Camp, Huub J M
AU  - Op den Camp HJ
FAU - Verweij, Paul E
AU  - Verweij PE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antigens, Fungal)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Antigens, Fungal/*analysis
MH  - Aspergillosis/*diagnosis
MH  - Aspergillus/*immunology
MH  - Bifidobacterium/*immunology
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay
MH  - False Positive Reactions
MH  - Humans
MH  - Infant, Newborn
MH  - Lipopolysaccharides/*immunology
PMC - PMC1233979
EDAT- 2005/08/06 09:00
MHDA- 2005/09/16 09:00
PMCR- 2006/02/01
CRDT- 2005/08/06 09:00
PHST- 2005/08/06 09:00 [pubmed]
PHST- 2005/09/16 09:00 [medline]
PHST- 2005/08/06 09:00 [entrez]
PHST- 2006/02/01 00:00 [pmc-release]
AID - 43/8/3925 [pii]
AID - 2080-04 [pii]
AID - 10.1128/JCM.43.8.3925-3931.2005 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2005 Aug;43(8):3925-31. doi: 10.1128/JCM.43.8.3925-3931.2005.