PMID- 16084645
OWN - NLM
STAT- MEDLINE
DCOM- 20051021
LR  - 20181113
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 387
IP  - 2
DP  - 2005 Oct 21
TI  - Integration of engrafted Schwann cells into injured peripheral nerve: axonal 
      association and nodal formation on regenerated axons.
PG  - 85-9
AB  - Transplantation of myelin-forming cells can remyelinate axons, but little is 
      known of the sodium channel organization of axons myelinated by donor cells. 
      Sciatic nerve axons of female wild type mice were transected by a crush injury 
      and Schwann cells (SCs) from green fluorescence protein (GFP)-expressing male 
      mice were transplanted adjacent to the crush site. The male donor cells were 
      identified by GFP fluorescence and fluorescence in situ hybridization (FISH) for 
      Y chromosome. In nerves of GFP-expressing mice, GFP was observed in the axoplasm 
      and in the cytoplasmic compartments of the Schwann cells, but not in the myelin. 
      Following transplantation of GFP-SCs into crushed nerve of wild type mice, 
      immuno-electron microscopic analysis indicated that GFP was observed in the 
      cytoplasmic compartments of engrafted Schwann cells which formed myelin. Nodal 
      and paranodal regions of the axons myelinated by the GFP-SCs were identified by 
      Na(v)1.6 sodium channel and Caspr immunostaining, respectively. Nuclear 
      identification of the Y chromosome by FISH confirmed the donor origin of the 
      myelin-forming cells. These results indicate that engrafted GFP-SCs participate 
      in myelination of regenerated peripheral nerve fibers and that Na(v)1.6 sodium 
      channel, which is the dominant sodium channel at normal nodes, is reconstituted 
      on the regenerated axons.
FAU - Radtke, Christine
AU  - Radtke C
AD  - Department of Neurology, LCI 7, P.O. Box 208018, Yale University School of 
      Medicine, New Haven, CT 06516, USA.
FAU - Akiyama, Yukinori
AU  - Akiyama Y
FAU - Lankford, Karen L
AU  - Lankford KL
FAU - Vogt, Peter M
AU  - Vogt PM
FAU - Krause, Diane S
AU  - Krause DS
FAU - Kocsis, Jeffery D
AU  - Kocsis JD
LA  - eng
GR  - R01 NS043432/NS/NINDS NIH HHS/United States
GR  - R01 NS043432-05/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Cntnap1 protein, mouse)
RN  - 0 (NAV1.6 Voltage-Gated Sodium Channel)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Scn8a protein, mouse)
RN  - 0 (Sodium Channels)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Cell Adhesion Molecules, Neuronal/metabolism
MH  - Cell Compartmentation/physiology
MH  - Cytoplasm/metabolism/ultrastructure
MH  - Disease Models, Animal
MH  - Female
MH  - Green Fluorescent Proteins/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Transmission
MH  - Myelin Sheath/*physiology/ultrastructure
MH  - NAV1.6 Voltage-Gated Sodium Channel
MH  - Nerve Regeneration/*physiology
MH  - Nerve Tissue Proteins/metabolism
MH  - *Peripheral Nerve Injuries
MH  - Peripheral Nerves/surgery/ultrastructure
MH  - Ranvier's Nodes/*physiology/ultrastructure
MH  - Schwann Cells/physiology/*transplantation/ultrastructure
MH  - Sciatic Neuropathy/metabolism/physiopathology/*therapy
MH  - Sodium Channels/metabolism
MH  - Y Chromosome/genetics/metabolism
PMC - PMC2605373
MID - NIHMS80442
EDAT- 2005/08/09 09:00
MHDA- 2005/10/22 09:00
PMCR- 2008/12/18
CRDT- 2005/08/09 09:00
PHST- 2005/01/18 00:00 [received]
PHST- 2005/06/21 00:00 [revised]
PHST- 2005/06/24 00:00 [accepted]
PHST- 2005/08/09 09:00 [pubmed]
PHST- 2005/10/22 09:00 [medline]
PHST- 2005/08/09 09:00 [entrez]
PHST- 2008/12/18 00:00 [pmc-release]
AID - S0304-3940(05)00760-3 [pii]
AID - 10.1016/j.neulet.2005.06.073 [doi]
PST - ppublish
SO  - Neurosci Lett. 2005 Oct 21;387(2):85-9. doi: 10.1016/j.neulet.2005.06.073.