PMID- 16084877
OWN - NLM
STAT- MEDLINE
DCOM- 20051227
LR  - 20061115
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 84
IP  - 2
DP  - 2005 Aug
TI  - Fluorescence in situ hybridization sperm analysis of six translocation carriers 
      provides evidence of an interchromosomal effect.
PG  - 365-73
AB  - OBJECTIVE: To investigate the existence of an interchromosomal effect in balanced 
      reciprocal translocation carriers and to evaluate their risk of having an 
      affected child or repeated spontaneous abortions. DESIGN: Analysis of chromosomal 
      sperm content by fluorescence in situ hybridization (FISH) using probes for 
      chromosomes 1, 15, 16, 17, 18, X, and Y. SETTING: University hospital. 
      PATIENT(S): Six male carriers of balanced chromosome rearrangements, one with 
      normal sperm parameters and five with oligoasthenoteratozoospermia, and seven 
      fertile controls. INTERVENTION(S): Fluorescence in situ hybridization for 
      chromosomal enumeration on sperm samples. MAIN OUTCOME MEASURE(S): Rate of disomy 
      for the studied chromosomes. RESULT(S): A total of 123,842 spermatozoa were 
      scored (82,181 for controls and 41,661 for patients). For each patient, at least 
      one chromosome studied presented a significantly increased rate of disomy. One 
      patient showed a clear interchromosomal effect for at least three of the six 
      chromosomes studied. Disomy rates were statistically significantly and inversely 
      correlated with the total progressive motility of spermatozoa. CONCLUSION(S): The 
      observed interchromosomal effect seems to be translocation, patient, and 
      chromosome dependent. Variable effects were observed, according to the chromosome 
      studied. When we looked at patients carrying the same translocation, the level of 
      disomy rate was variable and affected different chromosomes.
FAU - Machev, Nadejda
AU  - Machev N
AD  - Service de Biologie de la Reproduction, SIHCUS-CMCO, CHU de Strasbourg, 
      Schiltigheim, France.
FAU - Gosset, Philippe
AU  - Gosset P
FAU - Warter, Stephanie
AU  - Warter S
FAU - Treger, Michele
AU  - Treger M
FAU - Schillinger, Monique
AU  - Schillinger M
FAU - Viville, Stephane
AU  - Viville S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - Chi-Square Distribution
MH  - *Chromosome Mapping
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Oligospermia/genetics
MH  - Spermatozoa/cytology/*physiology
MH  - Translocation, Genetic/*genetics
MH  - Uniparental Disomy
EDAT- 2005/08/09 09:00
MHDA- 2005/12/28 09:00
CRDT- 2005/08/09 09:00
PHST- 2004/07/15 00:00 [received]
PHST- 2005/03/04 00:00 [revised]
PHST- 2005/03/04 00:00 [accepted]
PHST- 2005/08/09 09:00 [pubmed]
PHST- 2005/12/28 09:00 [medline]
PHST- 2005/08/09 09:00 [entrez]
AID - S0015-0282(05)00850-2 [pii]
AID - 10.1016/j.fertnstert.2005.03.026 [doi]
PST - ppublish
SO  - Fertil Steril. 2005 Aug;84(2):365-73. doi: 10.1016/j.fertnstert.2005.03.026.